Evaluation of Sedative and Anxiolytic Activity of Methanolic Extract of Lablab purpureus (L.) Sweet Seeds in Experimental Mice Model
Joushan Ara *
Department of Pharmacy, University of Science and Technology Chittagong (USTC), Chittagong 4202, Bangladesh.
Md. Rabiul Hossain
Department of Pharmacy, University of Science and Technology Chittagong (USTC), Chittagong 4202, Bangladesh.
Md. Saeedul Alam Nizami
Department of Pharmacy, University of Science and Technology Chittagong (USTC), Chittagong 4202, Bangladesh.
Abu Sadat Md. Sayem
Department of Pharmacy, University of Science and Technology Chittagong (USTC), Chittagong 4202, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Aims: Lablab purpureus (Fabaceae) a well-known Bangladeshi vegetable combines a great number of qualities with its adaptability and functional food properties. But still there is no evidance based report on neuropharmacological activities of Lablab purpureus seeds. For this reason, the present study was designed to analyze in vivo CNS effect of methanolic extract of Lablab purpureus seeds.
Place and Duration of Study: Department of Pharmacy, USTC between April 2017 and May 2018.
Methodology: In this study, the crude seeds extract of Lablab purpureus was evaluated for its sedative and anxiolytic effect using animal behavioral models, such as open field and hole cross tests for its sedative properties and an elevated plus maze (EPM) test for its anxiolytic potential.
Results: In open field and hole cross test, the seeds extract of Lablab purpureus showed a dose dependent suppression of motor activity and exploratory behaviour. In EPM test, animals treated with higher dose of methanolic extract showed significant (p<0.01) increased exploration to and time spent in EPM open arms.
Conclusion: This study provide in vivo evidence that the methanolic extract of Lablab purpureus has significant sedative and anxiolytic activity. Further studies on active constituent of the extract can provide approaches for therapeutic intervention.
Keywords: Elevated plus maze, hole cross, Lablab purpureus, neuropharmacology, open field.