Evaluation of the Effects of Aqueous Extract of Sarcocephalus latifolius on Sexual Behaviour in Male Rats
European Journal of Medicinal Plants,
Page 23-32
DOI:
10.9734/ejmp/2023/v34i11117
Abstract
Background: Sarcocephalus latifolius, a plant of the Rubiaceae family, is found exclusively in tropical Africa, more particularly in sub-Saharan West Africa, stretching from Senegal to Zaire, but is also found as far east as Sudan (Sudan-Guinean zone). The leaves of this plant are used in traditional African medicine to treat numerous pathologies such as infertility.
Aims/Objective: The present study aims to evaluate the effects of the aqueous extract of Sarcocephalus latifolius leaves on sexual parameters in male rats.
Methods: The sexual parameters (latency of the mating position, latency of intromission, latency of ejaculation and mean copulation interval) were assessed by the classical method by administering distilled water, sildenafil citrate (reference molecule) called Viagra at the dose of 5 mg/kg of body weight and the aqueous extract of Sarcocephalus latifolius leaves at the dose of 1000 mg/kg of body weight per day for eight days to three batches of male rats respectively. These parameters were determined over a period of thirty minutes.
Results: The results obtained show that the aqueous extract of Sarcocephalus latifolius leaves at a dose of 1000 mg/kg of body weight significantly (P < .001) decreases the latency time of mating position and the latency time of intromission, whereas this extract significantly (P < .001) increases the latency time of ejaculation and the average copulation interval.
Conclusion: Aqueous extract of Sarcocephalus latifolius leaves has aphrodisiac properties which are due to sterols, flavonoids and saponosides.
Keywords:
- Sarcocephalus latifolius
- aphrodisiac
- sildenafil citrate
How to Cite
Yaya, S. T., Louise, A. B. M., Mamadou, D., Koffi, S., & Sirabana, C. (2023). Evaluation of the Effects of Aqueous Extract of Sarcocephalus latifolius on Sexual Behaviour in Male Rats. European Journal of Medicinal Plants, 34(1), 23-32. https://doi.org/10.9734/ejmp/2023/v34i11117
References
WHO. Manual for the standardized investigation, diagnostic and management of themale. The baths press bath. 2000;91.
Comhaire FH. Male infertility: Clinical investigations, cause evaluation and treatment. Ed. Chapman and Hall, Alden Press oxford. 1996;395.
Mulhall JP, Bukofzer S, Edmonds AL, George M. An open-label, uncontrolled dose optimization study of sublingual apomorphine in erectile dysfunction. Clinical Therapy. 2001;23:1260-1271.
Hilaly JE, Israili ZH, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals. Journal of Ethnopharmacology. 2004;91:43-50.
Ouakrouch IA, Amal S, Akhdari N, Hocar O. Enquête ethnobotanique à propos des plantes médicinales utilisées dans le traitement traditionnel du vitiligo à Marrakech, Maroc. Annales de Dermatologie et de Vénéréologie. 2017;144(12):S334.
Dossou AJ, Fandohan AB. Utilisation des plantes médicinales pour prévenir et guérir les morsures de serpents: état des lieux et perspectives (synthèse bibliographique). Biotechnologie, Agronomie, Société et Environnement (BASE). 2021;25(2):57-70.
Gill LS. Ethnomedical uses of plants in Nigeria. Benin, Nigeria: Uniben Press. 1992;77.
Okafor J, Ham R. Identification, utilisation et conservation des plantes médicinales dans le sud-est du Nigéria. Thème Biodiversité Africaine. 1999;3:1-8.
N’guessan K, Kouadio K, Kouamé NF. Plantes emménagogues utilisées en médecine traditionnelle par les peuples Abbey et Krobou d’Agboville (Côte d’ivoire) Pharmacopée et Médecine Traditionnelle Africaine. 2006;14:137-158.
Mbaïhingam D, DJekota C, Bakarnga-via I. Testicular hernia and herbal medicine in Dodinda and Ngondong in western Logone occidental province in Chad. GSC Advanced Research and Reviews, 2022;13(02):091-097.
DOI:https://doi.org/10.30574/gscarr.2022.13.2.0204
Nurudeen Q, Salimon S, Falana M, Yakubu M, Akanji M. Ethnopharmacological Survey of Plants Used for the Treatment of Female Sexual Dysfunction and Infertility in Ilorin, Nigeria. Traditional And Integrative Medicine. 2022;7(1):104-123.
Botineau M. Botanique systématique et appliquée des plantes à fleurs. Lavoisier Edition. 2010;1403.
Anonyme. Council Directive of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Communities, 1986;358:1-28.
Bekro YA, Bekro JAM, Boua BB, Tra BFH, Ehile EE. Etude ethnobotanique et screening phytochimique de Caesalpinia benthamiana (Baill.) (Caesalpiniaceae). Revue. Sciences &. Nature. 2007;4(2):217-225.
OECD. Test guidline 423, OECD guidline for chemicals. Acute oral toxicity-acute toxic class method. 2001;14.
Cariton AE. Experimental surgery of the genital system. In: William IG, James EH (eds) Methods of animal experimentation: research surgery and care of the research animal; Part B Surgical approaches to organ systems. Academic Press, Inc., Orlando, Florida. 1986;191.
Ratnasooriya DW, Dharmasiri MG. Effects of Terminalia catappa seeds on sexual behaviour and fertility of male rats. Asian Journal of Andrology. 2000;2: 213-219.
Szechtman H, Hershkowitz M, Simantov R. Sexual behavior decreases pain sensitivity and stimulates endogenous opioids in male rats. European Journal of Pharmacology. 1981;70(3):279-285.
Carro-Jua´reza M, Cervantesa E, Cervantes-Me´ndeza M, Rodrı´guez-Manz G. Aphrodisiac properties of Montanoa tomentosa aqueous crude extract in male rats. Pharmacology, Biochemistry and Behavior. 2004;78:129-134.
Mbongue FGY. Etude des propriétés et des effets de certaines plantes de la flore camerounaise sur le comportement sexuel des rats adultes .Thèse de Doctorat PhD. Université de Yaoundé I, Cameroun. 222. Metabolism of testosterone. Recent prog. Horm. Res. 2003;22:245-281.
Edagha IA, Atting IA, Bassey EI, Ukpe SJ. Erythropoietic and Hepatoprotective Potential of Ethanolic Extract of Nauclea latifolia in Mice Infected with Plasmodium berghei berghei. American Journal of Medical Sciences and Medicine. 2014;2(1):7-12.
DOI: 10.12691/ajmsm-2-1-2
Iheagwam F, Israel E, Kayode K, De Campos O, Ogunlana O, Chinedu SN. Bioactive screening and in vitro antioxidant assessment of Nauclea latifolia leaf decoction. AIP Conference Proceedings. 2018;1954(1):030015.
Oyedeji-Amusa MO, Ashafa AOT. Medicinal properties of whole fruit extracts of Nauclea latifolia Smith.: Antimicrobial, antioxidant and hypoglycemic assessments. South African Journal of Botany. 2019;121:105-113.
Available :https://doi.org/10.1016/j.sajb.2018.11.001
Koko DIKE, Djego J, Gbenou J, Hounzangbe-Adote SM, Sinsin B. Etude phytochimique des principales plantes galactogènes et emménagogues utilisées dans les terroirs riverains de la Zone cynégétique de la Pendjari. International Journal of Biological and Chemical Sciences. 2018;5(2):618-633.
Chukwudozie KI, Ezeudoka BC, Okechukwu VC. Hepatoprotective Properties of Sarcocephalus latifolius Extract in Hyperglycemic Rat Model. Journal of Drug Delivery & Therapeutics. 2022;12(2-s):72-76.
DOI: http://dx.doi.org/10.22270/jddt.v12i2-s.5268
Yakubu MT, Akanji MA, Oladiji AT. Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials. Pharmacognosy Review. York. 2007;1:49-56, 99-142.
Yakubu MT, Afolayan AJ. Effect of aqueous extract of Bulbine natalensis (Baker) stem on the sexual behavior of male rats. International journal of Andrology. 2009;32(6):629-36.
DOI: 10.1111/j.1365-2605.2008.00910.x
Morabandza CJ, Ondele R, Elion Itou RDG, Etou Ossibi AW, Imbiella C, Mokondjimobe E, Ongoka PR, Abena AA. Aphrodisiac activity of aqueous and hydro ethanolic extracts of the stem bark of strychnos camptoneura (longaniaceae) in wistar rat. Asian Journal of Science and Technology. 2017;08(10):6055- 6059.
Beach FA. Sexual attractivity, perceptivity and receptivity, Hormones and Behavior. 1976;7:105-138.
Hooi HA, Meng KS. Eurycoma longifolia Jack enhances libido in sexually experience male rats. Experimental Animals. 1997;46(4):287-290.
Haroldo AT, Fernanda BM P, Saiprasad M Z, Edson A, Cleber H J. Endocrinologie. 3éme Ed. 254-268 health perspectives. 1989;103(7):59-62.
Gayatri G, Dheeraj HN, Mithilesh R, Harsh G. Pharmacological evaluation of ayurvedic plants for aphrodisiac activity in experimental animals. Journal of Complementary and Integrative Medicine. 2010; 7: 1.
Available:https://doi.org/10.2202/1553-3840.1418
Adimoelja A. Phytochemicals and the breakthrough of traditional herbs in the management of sexual dysfunctions. International Journal of Andrology. 2000;2:82-84.
DOI: 10.1046/j.1365-2605.2000.00020.x
Quasie O, Martey ONK, Nyarko AK, Gbewonyoand WSK, Okine LKN. Modulation of penile erection in rabbits by mondia whitei: Possible mechanism of action. African Journal of Traditional, Complementary and Alternative Medecine. 2010;7(3):241-252.
-Akassa H, Ondele R, Peneme BML, Etou Ossibi AW, Morabandza CJ, Tamboura HH, Abena AA. Activité aphrodisiaque et étude du mécanisme d’action de l’extrait aqueux des écorces de tronc de Pausinystalia yohimbe Kschum (Rubiaceae) chez le rat wistar. Journal of Animal & Plant Sciences. 2019;39:6372-6383.
Gauthaman K, Adaikan P. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction: an evaluation using primates, rabbit and rat. Phytomedicine. 2008; 15(1-2):44-54.
Saleem A, Ahotupa M, Pihlaja K. Total phenolics concentration and antioxidant properties of extracts of medicinal plants of Pakistan. Zeitschrift Naturforschung. 2001;56(11-12):973-978.
Esimone CO, Omobuwajo OR, Sowemimo AA, Proksch P. Single-cycle vector based antiviral screening assays. Journal of Animal & Plant Sciences. 2006;39(1):6372-6383.
Drewes SE, Georges J, Khan FR. Recent findings on natural products with erectile dysfunction activity. Phytochemistry. 2003; 62:1019-1025.
Comhaire FH. Male infertility: Clinical investigations, cause evaluation and treatment. Ed. Chapman and Hall, Alden Press oxford. 1996;395.
Mulhall JP, Bukofzer S, Edmonds AL, George M. An open-label, uncontrolled dose optimization study of sublingual apomorphine in erectile dysfunction. Clinical Therapy. 2001;23:1260-1271.
Hilaly JE, Israili ZH, Lyoussi B. Acute and chronic toxicological studies of Ajuga iva in experimental animals. Journal of Ethnopharmacology. 2004;91:43-50.
Ouakrouch IA, Amal S, Akhdari N, Hocar O. Enquête ethnobotanique à propos des plantes médicinales utilisées dans le traitement traditionnel du vitiligo à Marrakech, Maroc. Annales de Dermatologie et de Vénéréologie. 2017;144(12):S334.
Dossou AJ, Fandohan AB. Utilisation des plantes médicinales pour prévenir et guérir les morsures de serpents: état des lieux et perspectives (synthèse bibliographique). Biotechnologie, Agronomie, Société et Environnement (BASE). 2021;25(2):57-70.
Gill LS. Ethnomedical uses of plants in Nigeria. Benin, Nigeria: Uniben Press. 1992;77.
Okafor J, Ham R. Identification, utilisation et conservation des plantes médicinales dans le sud-est du Nigéria. Thème Biodiversité Africaine. 1999;3:1-8.
N’guessan K, Kouadio K, Kouamé NF. Plantes emménagogues utilisées en médecine traditionnelle par les peuples Abbey et Krobou d’Agboville (Côte d’ivoire) Pharmacopée et Médecine Traditionnelle Africaine. 2006;14:137-158.
Mbaïhingam D, DJekota C, Bakarnga-via I. Testicular hernia and herbal medicine in Dodinda and Ngondong in western Logone occidental province in Chad. GSC Advanced Research and Reviews, 2022;13(02):091-097.
DOI:https://doi.org/10.30574/gscarr.2022.13.2.0204
Nurudeen Q, Salimon S, Falana M, Yakubu M, Akanji M. Ethnopharmacological Survey of Plants Used for the Treatment of Female Sexual Dysfunction and Infertility in Ilorin, Nigeria. Traditional And Integrative Medicine. 2022;7(1):104-123.
Botineau M. Botanique systématique et appliquée des plantes à fleurs. Lavoisier Edition. 2010;1403.
Anonyme. Council Directive of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Communities, 1986;358:1-28.
Bekro YA, Bekro JAM, Boua BB, Tra BFH, Ehile EE. Etude ethnobotanique et screening phytochimique de Caesalpinia benthamiana (Baill.) (Caesalpiniaceae). Revue. Sciences &. Nature. 2007;4(2):217-225.
OECD. Test guidline 423, OECD guidline for chemicals. Acute oral toxicity-acute toxic class method. 2001;14.
Cariton AE. Experimental surgery of the genital system. In: William IG, James EH (eds) Methods of animal experimentation: research surgery and care of the research animal; Part B Surgical approaches to organ systems. Academic Press, Inc., Orlando, Florida. 1986;191.
Ratnasooriya DW, Dharmasiri MG. Effects of Terminalia catappa seeds on sexual behaviour and fertility of male rats. Asian Journal of Andrology. 2000;2: 213-219.
Szechtman H, Hershkowitz M, Simantov R. Sexual behavior decreases pain sensitivity and stimulates endogenous opioids in male rats. European Journal of Pharmacology. 1981;70(3):279-285.
Carro-Jua´reza M, Cervantesa E, Cervantes-Me´ndeza M, Rodrı´guez-Manz G. Aphrodisiac properties of Montanoa tomentosa aqueous crude extract in male rats. Pharmacology, Biochemistry and Behavior. 2004;78:129-134.
Mbongue FGY. Etude des propriétés et des effets de certaines plantes de la flore camerounaise sur le comportement sexuel des rats adultes .Thèse de Doctorat PhD. Université de Yaoundé I, Cameroun. 222. Metabolism of testosterone. Recent prog. Horm. Res. 2003;22:245-281.
Edagha IA, Atting IA, Bassey EI, Ukpe SJ. Erythropoietic and Hepatoprotective Potential of Ethanolic Extract of Nauclea latifolia in Mice Infected with Plasmodium berghei berghei. American Journal of Medical Sciences and Medicine. 2014;2(1):7-12.
DOI: 10.12691/ajmsm-2-1-2
Iheagwam F, Israel E, Kayode K, De Campos O, Ogunlana O, Chinedu SN. Bioactive screening and in vitro antioxidant assessment of Nauclea latifolia leaf decoction. AIP Conference Proceedings. 2018;1954(1):030015.
Oyedeji-Amusa MO, Ashafa AOT. Medicinal properties of whole fruit extracts of Nauclea latifolia Smith.: Antimicrobial, antioxidant and hypoglycemic assessments. South African Journal of Botany. 2019;121:105-113.
Available :https://doi.org/10.1016/j.sajb.2018.11.001
Koko DIKE, Djego J, Gbenou J, Hounzangbe-Adote SM, Sinsin B. Etude phytochimique des principales plantes galactogènes et emménagogues utilisées dans les terroirs riverains de la Zone cynégétique de la Pendjari. International Journal of Biological and Chemical Sciences. 2018;5(2):618-633.
Chukwudozie KI, Ezeudoka BC, Okechukwu VC. Hepatoprotective Properties of Sarcocephalus latifolius Extract in Hyperglycemic Rat Model. Journal of Drug Delivery & Therapeutics. 2022;12(2-s):72-76.
DOI: http://dx.doi.org/10.22270/jddt.v12i2-s.5268
Yakubu MT, Akanji MA, Oladiji AT. Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials. Pharmacognosy Review. York. 2007;1:49-56, 99-142.
Yakubu MT, Afolayan AJ. Effect of aqueous extract of Bulbine natalensis (Baker) stem on the sexual behavior of male rats. International journal of Andrology. 2009;32(6):629-36.
DOI: 10.1111/j.1365-2605.2008.00910.x
Morabandza CJ, Ondele R, Elion Itou RDG, Etou Ossibi AW, Imbiella C, Mokondjimobe E, Ongoka PR, Abena AA. Aphrodisiac activity of aqueous and hydro ethanolic extracts of the stem bark of strychnos camptoneura (longaniaceae) in wistar rat. Asian Journal of Science and Technology. 2017;08(10):6055- 6059.
Beach FA. Sexual attractivity, perceptivity and receptivity, Hormones and Behavior. 1976;7:105-138.
Hooi HA, Meng KS. Eurycoma longifolia Jack enhances libido in sexually experience male rats. Experimental Animals. 1997;46(4):287-290.
Haroldo AT, Fernanda BM P, Saiprasad M Z, Edson A, Cleber H J. Endocrinologie. 3éme Ed. 254-268 health perspectives. 1989;103(7):59-62.
Gayatri G, Dheeraj HN, Mithilesh R, Harsh G. Pharmacological evaluation of ayurvedic plants for aphrodisiac activity in experimental animals. Journal of Complementary and Integrative Medicine. 2010; 7: 1.
Available:https://doi.org/10.2202/1553-3840.1418
Adimoelja A. Phytochemicals and the breakthrough of traditional herbs in the management of sexual dysfunctions. International Journal of Andrology. 2000;2:82-84.
DOI: 10.1046/j.1365-2605.2000.00020.x
Quasie O, Martey ONK, Nyarko AK, Gbewonyoand WSK, Okine LKN. Modulation of penile erection in rabbits by mondia whitei: Possible mechanism of action. African Journal of Traditional, Complementary and Alternative Medecine. 2010;7(3):241-252.
-Akassa H, Ondele R, Peneme BML, Etou Ossibi AW, Morabandza CJ, Tamboura HH, Abena AA. Activité aphrodisiaque et étude du mécanisme d’action de l’extrait aqueux des écorces de tronc de Pausinystalia yohimbe Kschum (Rubiaceae) chez le rat wistar. Journal of Animal & Plant Sciences. 2019;39:6372-6383.
Gauthaman K, Adaikan P. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction: an evaluation using primates, rabbit and rat. Phytomedicine. 2008; 15(1-2):44-54.
Saleem A, Ahotupa M, Pihlaja K. Total phenolics concentration and antioxidant properties of extracts of medicinal plants of Pakistan. Zeitschrift Naturforschung. 2001;56(11-12):973-978.
Esimone CO, Omobuwajo OR, Sowemimo AA, Proksch P. Single-cycle vector based antiviral screening assays. Journal of Animal & Plant Sciences. 2006;39(1):6372-6383.
Drewes SE, Georges J, Khan FR. Recent findings on natural products with erectile dysfunction activity. Phytochemistry. 2003; 62:1019-1025.
-
Abstract View: 39 times
PDF Download: 19 times
