Effects of Dolutegravir (DTG) on Survival, Pupariation and Emergence in Drosophila melanogaster: The Rescue Role of Brassica oleracea
European Journal of Medicinal Plants,
The study aimed at determining the protective role of Brassica oleracea on dolutegravir-induced changes in Pupariation and Emergence of Drosophila melanogaster. D. melanogaster aged 3-5 days old were exposed to different concentrations (0.5 to 4 mg/ 5 g diet) of dolutegravir and B. oleracea extract (7.5–1000 mg/5 g diet) for 7 days to determine the lethal concentration (LC50). D. melanogaster were then exposed to the extract (50, 100, 200, and 400 mg/5 g diet) and controls (diet alone and vitamin C) to assess their effects on pupariation and emergence. A 14-day assay was also performed to evaluate the effect of the extract and toxicant (dolutegravir) on fly survival. The result showed a dose-dependent significant decrease (P < 0.05) and a dose-dependent significant increase (P < 0.05) in survival for D. melanogaster exposed to dolutegravir and the extract respectively, when compared to the control group. Results showed a delay in pupariation and decrease in mean pupariation in flies exposed to dolutegravir alone. An improvement in the same parameters was observed in D. melanogaster pre-treated with the extract before exposure to dolutegravir. D. melanogaster pre-treated with 200 and 400 mg extract per 5 g diet showed emergence that was comparable to those in the control groups. A significant decrease (P < 0.05) was observed in the groups exposed to 50 and 100 mg extract per 5 g diet, suggesting no protection at these doses. This study concludes that B. oleracea leaf extract, at certain concentrations, is able to protect against dolutegravir-induced changes in pupariation and emergence in D. melanogaster.
- Brassica oleracea
How to Cite
Parvathi VD, Amritha AS, Paul SFD. Wonder animal model for genetic studies– Drosophila melanogaster–its life cycle and breeding methods Sri Ramachandra. J. Med. 2009;2:33-38.
Chiang HC, Hodson AC. An analytical study of population growth in Drosophila melanogaster. Ecological Monographs. 1950;20(3):173-206.
Riedl CAL, Riedl M, Mackay TFC, Sokolowski MB. Genetic and behavioral analysis of natural variation in Drosophila melanogaster pupation position. 2007;Fly1: 23–32.
Joshi A, Mueller LD. Directional and stabilizing density-dependent natural-selection for pupation height in Drosophila melanogaster. Evolution. 1993;47: 176–184.
Sokolowski MB, Bauer SJ. Genetic analysis of pupation distance in Drosophila melanogaster. Heredity. 1989;62:177–183.
Hodge S, Caslaw P. The effect of resource pH on pupation height in Drosophila (Diptera: Drosophilidae). Journal of Insect Behavior. 1998;11:47–57.
Seyahooei MA, Kraaijeveld-Smit FJL, Kraaijeveld K, Crooijmans JBM, Van Dooren TJM et al. Closely related parasitoids induce different pupation and foraging responses in Drosophila larvae. Oikos. 2009;118:1148–1157.
Manning M, Markow TA. Light-dependent pupation site preferences in Drosophila. 2. Drosophila melanogaster and Drosophila-simulans. Behavior Genetics. 1981;11: 557–563.
Rodriguez L, Sokolowski MB, Shore JS. Habitat selection by Drosophila melanogaster larvae. Journal of Evolutionary Biology. 1992;5:61–70.
Adams JL, Greener BN&Kashuba AD. Pharmacology of HIV Integrase Inhibitors. Current Opinion on HIV AIDS. 2012; 7(5):390-400.
Sayanti P, Sumedha, R. Study of the changes in life cycle parameters of Drosophila melanogaster exposed to fluorinated insecticide, cryolite. Toxicology and Industrial Health Journal. 2013;31(12).
Sanlier N, Guler SM. The benefits of Brassica vegetables on Human Health. J Human Health Res. 2018;1:104.
Fristrom, D, Fristrom JW. The Metamorphic Development of the Adult Epidermis. In: The Development of Drosophila melanogaster. Cold Spring Harbor Laboratory Press, Plainview New York.1993;843-897.
Mohammad F, Singh P. A drosophila systems model of pentylenetetrazole induced locomotor plasticity responsive to antiepileptic drugs; 2009.
Charpentier G, Louat F, Bonmatin JM. Lethal and sublethal effects of imidacloprid, after chronic exposure, on the insect model Drosophila melanogaster. Environ Sci Technol. 2014;48(7): 4096- 4102.
Abolaji AO, Kamdem JP, Lugokenski T. Henrique, et al. Involvement of oxidative stress in 4-vinylcyclohexene- induced toxicity in Drosophila melanogaster. Free Radic Biol Med. 2014;71: 99-108.
Abbas ZK, Saggu S, Sakeran MI et al. Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichoriumintybus L.) leaves. Saudi J Biol Sci. 2015;22:322–6.
Shi J, Yu J, Pohorly JE, Kakuda Y. Polyphenolics in grape seeds— biochemistry and functionality. J Med Food. 2003;6:291–9.
Wang H, Cao G, Prior RL. Total antioxidant capacity of fruits. J Agric Food Chem. 1996;44:701–5. 21.
Loomis TA, Hayes AW. Loomis’s essentials of toxicology. 4. California: Academic press. 1996; 208–245.
Gargano JW, Martin I, Bhandari P, Grotewiel MS. Rapid iterative negative geotaxis (RING): A new method for assessing age-related locomotor decline in Drosophila. Experimental gerontology. 2005;40:386–395. [PubMed] [Google Scholar]
Rera M et al. Modulation of longevity and tissue homeostasis by the Drosophila PGC-1 homolog. Cell Metab. 2011;14: 623–634.
Hackney JF, Cherbas P. (2014) Injury response checkpoint and developmental timing in insects, Fly 2014;8:4: 226-231.
Mala J, Sehnal F, Kumaran AK, Granger NA (1987). Effects of starvation, chilling, and injury on endocrine gland function in Galleria mellonella. Arch. Insect Biochem. Physiol; 2014.
Lawrence T. Reiter, Lorraine Potocki, Sam Chien, Michael Gribskov and Ethan Bier. A systematic analysis of human disease associated gene sequences in Drosophila melanogaster; Genome Res. 2001;11:1114.
Iorjiim WM, Omale S, Bagu GD, Gyang SS. Alemika ET. Reproductive and oxidative stress toxicity of dolutegravirbased combination antiretroviral therapy in Drosophila melanogaster. J Adv Med Pharm Sci. 2020;22(6):26-40.
Abstract View: 71 times
PDF Download: 38 times