Open Access Case Report

Effect of Lemon Oil Concentrate Cymbopogon citratus ERILIM® towards the Helicobacter pylori Bacteria: A Case Report

J. C. Ruvalcaba Ledezma, J. Reynoso Vázquez, E. G. Olvera Hernández, L. O. Aguirre Rembao, J. A. Pérez Islas, E. A. Baltazar García, Y. J. García Cruz

European Journal of Medicinal Plants, Page 10-15
DOI: 10.9734/ejmp/2022/v33i730477

This research is based on the effect of the lemon tea concentrate whose polyphenols are powerful antioxidants. Studies have shown that they suppress free radicals in a more effective way than vitamins C or E; in some of cases, specifically in H. pylori, which generally colonizes the human stomach as a single strain with stable characteristics. Authors mention that this colonization can be stable throughout life. This bacterium is related to chronic gastritis, and although H. pylori infection is still the main cause of duodenal and gastric ulcers, the prevalence of ulcers associated with H. pylori is decreasing, while that of related ulcers with non-steroidal anti-inflammatory drugs (NSAIDs) is increasing. Much of what has been published lately about H. pylori and has focused on its important changes in the pattern of the disease and in the potential value of eradication therapy.

Open Access Original Research Article

Phytochemical, Antioxidant, Antibacterial and Antifungal Investigations of Thuja orientalis Cone

Vaskar Das, Vijeta Rai, Nishika Jaishee, Shilpi Ghosh

European Journal of Medicinal Plants, Page 1-9
DOI: 10.9734/ejmp/2022/v33i730475

Background of the Study: Thuja orientalis L. is an indigenous medicinal plant belonging to family Cupressaceae found in Darjeeling Himalayan region, Dooars and Tarai region of India. The plant cone has been used traditionally as medicine to treat various diseases, like bronchitis, bacterial skin infection, osteoarthritis, trigeminal neuralgia.

Aim: The aim of the present study is to evaluate the plant cone for phytochemical constinuents, and in vitro antioxidant, and antimicrobial properties.

Place and Duration of Study: All the experiments were done in the Department of Biotechnology, University of North Bengal, Darjeeling, West Bengal, 734013, India.

Methodology: Methanolic extract of T. orientalis cone was analyzed for phytochemicals by various biochemical methods. Antioxidant properties were analyzed by in vitro assays of DPPH, ABTS, NO and H2O2 scavenging. Antibacterial property was analyzed by agar well diffusion method and antifungal assay was monitored by radial growth bioassay.

Results: Methanolic extract of T. orientalis cone contained flavonoid, phenol, saponin, tannin, terpenoid and alkaloid. The extract showed significant in-vitro antioxidant and antimicrobial activity.

Conclusions: The study revealed that T. orientalis cone has potential as source of antioxidant, antibacterial and antifungal agents. Our further study is directed towards the isolation, and characterization of active compound from methanolic extract and evaluation of its potentiality against high blood sugar.

Open Access Original Research Article

Preparation of Angiotico-2 (A-2) an Electrohomeopathic Remedy, its TLC, FTIR, Pharmacognocalogical, Phytochemical, Chemical, Heavy Metal, Pesticide, Food Additives and Microbial Screening for Safe Usage

P. Suresh Babu, D. C. Bhavya, E. Siddalinga Murthy, B. Ramesha, M. P. S. Pujji, Achala Mogra, I. Chandrika, S. Naveen Kumar

European Journal of Medicinal Plants, Page 16-27
DOI: 10.9734/ejmp/2022/v33i730478

To evaluate phytochemical, heavy metal, pesticide, microbial assay, and also FTIR studies of Angiatico-2 (A-2) an Electrohomeopathic remedy to evaluate safety and efficacy for its usage.     Electrohomeopathy / Electropathy is one purely herbal medical system invented by Italian C. C. Mattie (1809-1896) has been practiced since the 1860s across the world. Electrohomeopathy has its own unique principles, plants selection, the process of remedies preparation, diagnosis, selection, and combination of drugs for different diseases, dosage, and treatment methods. In India, it is estimated that there are about 450-500 institutions imparting education and research, about 4.5 to 5 lacks practitioners are practicing and millions of population getting benefits currently. The government of India initiated steps to recognize Electrohomeopathy medical system under the constitution. Unfortunately, there is very limited scientific evidence to evaluate the safety, efficacy, phytochemical, and pharmacology studies in Electrohomeopathy. We prepared Angiatico-2 an Electrohomeopathic remedy as Mother Solution and D4 dilutions and evaluated it’s phytochemical screening, TLC, FTIR spectrum, Physical and Chemical nature, presence or absence of heavy metals, Pesticide, Food Additives and Microbial   under NABL standards for its safety usage. Electrohomeopathic herbal remedy Angiatico-2 prepared by using 7 non poisonous plants by Krauss method under standard condition and not shown any heavy metals, pesticide presence and also no microbial presence above the limit level of FSSAI standard. Present studies of Electrohomeopathic herbal remedy Angiatico-2 preparation and its screening in standard scientific laboratory condition,   TLC & FTIR studies may become reference standards for the manufacture of Angiatico-2 for bulk quantity at industrial level for. The present research investigations outcome with reproducibility may become standard markers or signatures to assess the quality and safe use of Angiatico-2.

Open Access Original Research Article

Evaluation of Physiochemical Properties, Thermal Behavior and Phytopharmaceutical Potential of Citrus aurantium’s Essential Oil

Vikas Jha, Fatimabi Shaikh, Nikita Tendulkar, Siddhartha Pandya, Bhakti Madaye, Esha Gupta, Farheen Khan, Navdeep Kaur, Tisha Jain, Stanford Almeida, Pratyusha Mane

European Journal of Medicinal Plants, Page 28-40
DOI: 10.9734/ejmp/2022/v33i730479

The study investigates the therapeutic potential of the Citrus aurantium var. amara essential oil extracted from the blossoms of the bitter orange plant by examining its chemical composition, thermal stability, and potency against infectious disease-causing pathogens.  Initially, the volatile components of the essential oil were evaluated by obtaining a chromatographic fingerprint using HPTLC and FTIR spectrum identification. Furthermore, a thermal profile of the essential oil was obtained using the thermogravimetric-differential thermal analysis and differential scanning calorimetric analysis. A predetermined set of antibiotic-resistant microorganisms were used to examine the antibacterial activity of the essential oil. Lastly, its anti-inflammatory activity was assessed using the albumin denaturation assay. The research concluded that the Citrus aurantium var. Amara essential oil exhibits potential therapeutic characteristics which can be further explored through in vivo studies.

Open Access Original Research Article

Soursop (Annona muricata L.) Fruit Peels as Source of Phenolic Constituents and Annonacin with Biological Activities

Glauciemar Del-Vechio Vieira, Julianna Oliveira de Lucas Xavier, Ana Lúcia dos Santos de Matos Araújo, Jéssica Leiras Mota Conegundes, Elita Scio Fontes, Orlando Vieira de Sousa

European Journal of Medicinal Plants, Page 41-52
DOI: 10.9734/ejmp/2022/v33i730480

Aims: In this study, chemical constituents and biological activities of the Annona muricata L. fruit peels were evaluated using methanol extract (MEAM) and hexane (HFAM), dichloromethane (DFAM), ethyl acetate (EFAM), and butanol (BFAM) fractions.

Place and Duration of Study: All the experiments were done in the Department of Pharmaceutical Sciences and Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, 36026-900, Brazil, between January 2012 and July 2016.

Methodology: Phytochemical screening (specific chemical reactions), total phenolic and flavonoid contents (Spectrophotometric methods) and chemical compounds were assessed (High performance liquid chromatography analysis). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), beta-carotene, and thiobarbituric acid assays. The inhibitory effect against digestive enzymes (lipase, α-amylase and α-glucosidase) was measured by spectrophotometric assays and and toxicity by the brine shrimp lethality bioassay.

Results: Tannins, flavonoids, coumarins, terpenes and steroids, saponins, and alkaloids were detected. EFAM had the highest values of total phenolic and flavonoids, while a similar compound to annonacin was found in MEAM by HPLC. EFAM was also more active in DPPH and FRAP assays, and HFAM was effective in inhibiting the linoleic acid oxidation and the malondialdehyde. MEAM and fractions blocked lipase, α-amylase and α-glucosidase, while HFAM and DFAM were toxic against Artemia salina.

Conclusion: The results showed that the A. muricata fruit peels have important biological effects, which can bring great benefits to human and animal health.