Open Access Original Research Article

Phytochemical and GC-MS Analysis of Dendrophthoe falcata (L.f.) Ettingsh Stem

N. H. Shahare, S. K. Bodele

European Journal of Medicinal Plants, Page 1-8
DOI: 10.9734/ejmp/2019/v29i430159

Dendrophthoe falcata (L.f.) Ettingsh is an important medicinal plant belonging to the family Loranthaceae. It is perennial, climbing and woody parasitic plant of family Loranthaceae. It is ethnomedicinally used for treating ulcers, asthma, impotence, paralysis skin diseases and wounds. In the present study, an attempt was made to investigate the phytochemical constituents present in the Dendrophthoe falcata (L.f.) Ettingsh stem. The preliminary phytochemical screening in different solvents extract was done by using standard methods. The phytochemical screening revealed the presence of carbohydrates, protein and amino acids, fixed oils and fats, saponins, sterols, alkaloids, phenols, tannins, flavonoids, anthocyanin and anthraquinones in different solvent extracts. GC-MS analysis was performed on the methanolic extract to find out the chemical constituents. The GC-MS study revealed the presence of about 51 bioactive compounds. Cyclopentasiloxane, Cyclohexasiloxane, Cycloheptasiloxane, Cyclooctasiloxane, Cyclononasiloxane, Phthalic acid, Hexadecanoic acid, Cyclodecasiloxane, Cyclononasiloxane, Tetracosane, Tetratriacontane, Benzoic acid, 1-Monolinoleoylglycerol trimethylsilyl ether, Octadecane, ç-Sitosterol are some of the major phytoconstituents reported from the sample. Further research work is needed on these identified compounds to analyse their mode of action and usefulness in phytopharmaceutical industries.

Open Access Original Research Article

In vitro Antioxidant and Anti-inflammatory Activities of the Flower Extracts of Hibiscus vitifolius L

D. Prabhakaran, A. Rajeshkanna, M. M. Senthamilselvi, S. Solomon

European Journal of Medicinal Plants, Page 1-8
DOI: 10.9734/ejmp/2019/v29i430160

Objective: To determine the antioxidant and anti-inflammatory activities of the solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L.

Methods: The flower extract assessed for antioxidant activity using the 1,1–diphenyl-2-picryl-hydrazile (DPPH) radical scavenging assay and the reduced power assay was performed using the Ferric Reducing Capacity (FRC) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) to test the production method of nitric oxide (NO).

Results: The solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L showed good antioxidant activity in the scavenging DPPH radicals and the FRC assay compared to the standard sample. This powder sample also showed good anti-inflammatory activity in cell viability (LPS induced PBMC) assay and nitric oxide (NO) assay.

 Conclusion: These results suggest that the powder sample extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L has substantial antioxidant and anti-inflammatory activity.

Open Access Original Research Article

Effect of Rhustox on Micropropagation of Scoparia dulcis L. through Leaf Explants Culture

Sayema Zeya, Maheshwar Prasad Trivedi

European Journal of Medicinal Plants, Page 1-14
DOI: 10.9734/ejmp/2019/v29i430162

Aim: To investigate the effect of Rhus toxicodendron (30CH) along with different compositions of phytohormones (Auxin and Cytokinin) on the basis of  growth and multiplication of explants under optimum temperature under in-vitro conditions.

Study Design: To establish and design the standard protocol for the in-vitro propagation through leaf explant of Scoparia dulcis  under stress of phytohormones and homeopathic medicine Rhus toxicodendron (30CH).

Place and Duration of Study: The plant materials were  procured from the Herbal Botanical Garden Patna Science College, Department of Botany, Patna University, Patna, Bihar. The experimental part was carried out in Plant Tissue Culture Laboratory, between December 2017 to August 2018  in Department of  Botany P.U. Patna.

Methodlogy: The sterilized leaf explants were inoculated into MS media fortified with different phytohormones  (Auxin and Cytokinin) and Rhus tox(30CH) under aseptic environmental conditions for the growth and development of callus, embryoids etc.

Result: The explants in MS medium supplemented with auxins phytohormones and Rhus tox(30CH) exhibited that IAA (0.10 to 2.0 mg/l) and BAP (0.10 to 2.5 mg/l)  induces green and compact calli. Whereas at 0.30mg/l of IAA and 0.50 mg/l BAP induced brown friable calli. 2,4-D (1.5 mg/l) and Kinetin (1.5-6.5mg/l) concentrations induced brown and friable calli. Rhus tox(30CH) (100 µl/100 ml) enhances  proliferation with 2,4-D and Kinetin (1.5/1.5 mg/l.).

Conclusion:  After 42 days of culture initiation and establishment the callus was 520.0±1.12 mg in the mixture of 2,4-D and Kinetin (1.5 mg/l) in Rhus tox free medium. Whereas weight of callus were found to be 1092±0.74 mg after 42 days in the same medium of 2, 4-D and Kinetin (1.5/5.5 mg/l) supplemented with Rhus tox (100 µl/100 ml). Hence, the investigation proponded that the Rhus tox (CH30) has increased the rate of callus development and plantlet regeneration.

Open Access Original Research Article

In vitro Antisalmonellal and Antioxidant Activities of Leaves Extracts of Tectona grandis L. F. (Verbenaceae)

Gabriel Tchuente Kamsu, Richard Simo Tagne, Siméon P. C. Fodouop, Louis-Claire Ndel Famen, Norbert Kodjio, Steve Endeguele Ekom, Donatien Gatsing

European Journal of Medicinal Plants, Page 1-13
DOI: 10.9734/ejmp/2019/v29i430164

Aim: Study aimed to evaluate the in vitro antisalmonellal and antioxidant properties of Tectona grandis, a medicinal plant commonly used in traditional Cameroonian medicine for the treatment of typhoid fever.

Study Design: In vitro Antimicrobial and antioxidant analyses of plant extract using established protocols.

Place and Duration of Study: Plant materiel collection at Loum (Moungo Division, Littoral region of Cameroon), identification at the National Herbarium of Cameroon, extraction of plant and all analyses at the Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, University of Dschang-Cameroon, between August 2018 and April 2019.

Methodology: In vitro antisalmonellal activity of Tectona grandis extracts was evaluated by the liquid microdilution method as well as their antioxidant activity using by standard methods of H2O2 trapping, FRAP, DPPH radical trapping and OH radical trapping. Quantification test of total phenols and flavonoids and phytochemical screening of extract were also done.

Results: The results showed that most of the Tectona grandis extracts had minimal inhibitory concentrations (MICs) ranging from 64 to 1024 μg/mL. Ethanolic extract, 70% ethanol extract and aqueous decoction are the most effective compared to aqueous extracts with activities between 64 and 128 μg/mL on the bacteria tested. Antioxidant tests showed that all extracts except macerated and aqueous infused extracts exhibited high DPPH trapping activities. The antioxidant activities of the extracts are proportionally linked in most cases to their flavonoid and total phenol contents at a concentration of 200 µg/mL. Aqueous decoction showed better H2O2 trapping activity at concentrations less than or equal to 25 µg/mL.

Conclusion: These results show that the ethanolic extract and decocted aqueous of Tectona grandis leaves can be used as an alternative to treat salmonellosis.

Open Access Original Research Article

Ethnobotanical Potential and Phytochemical Screening of Bombax ceiba L.

Parth Depani, Kamlesh Gadhvi, Suhas Vyas

European Journal of Medicinal Plants, Page 1-8
DOI: 10.9734/ejmp/2019/v29i430163

Traditional knowledge of medicinal plants is showing important and significant values to society. One of the important trees growing in the Panchmahal region, Bombax ceiba, has immersed medicinal values sited in few kinds of literature. Local populations utilizing various parts of Bombax ceiba as medicines are to be known scientifically through traditional inputs. The present study was undertaken to emphasis the secondary metabolites present in different parts of B. ceiba growing region in Panchmahal, district. Traditional information about plant/tree in generally available related to leaves, trunk, root, fruits etc. the current study deals with knowledge of flowers along with thereof B. ceiba growing in Panchmahal district. Investigation for qualitative analysis of thorn, petals, and androecium was carried out for the tree B. ceiba growing in Panchamahal district.