European Journal of Medicinal Plants 2021-01-20T13:09:28+00:00 European Journal of Medicinal Plants Open Journal Systems <p style="text-align: justify;"><strong>European Journal of Medicinal Plants (ISSN: 2231-0894)</strong> is dedicated to publish&nbsp;high quality papers (<a href="/index.php/EJMP/general-guideline-for-authors">Click here for Types of paper</a>)&nbsp;in all areas of medicinal plants research including plant growth and development, agronomic management, plant nutrition, plant physiology, cell biology, molecular biology, biotechnology, medicinal properties, phytochemical constituents, fitoterapia, pharmacognosy, essential oils, ehnopharmacology and phytomedicine. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> Sabicea brasiliensis Wernham: Antioxidant Activity, Proliferative Effect and Modulation of Vascular Adenine Nucleotides Metabolism 2021-01-20T13:09:28+00:00 Douglas Souza Oliveira Mikaelle Costa Correia Bruna Juber de Araújo Fernanda Cardoso da Silva Paula Marynella Alves Pereira Lima Marcos de Souza Gomes Thaise Gonçalves de Araújo Cristina Ribas Fürstenau <p><strong>Aims:</strong> Study addressed the antioxidant activity (AA) of <em>Sabicea brasiliensis</em> roots crude extract (CE), ethyl acetate (EAF), and hydro-methanolic (HMF) fractions, and its impact on cell viability and adenine nucleotide hydrolysis in vascular A7r5 cells.</p> <p><strong>Materials and Methods:</strong> AA of CE, EAF and HMF were determined by the inhibition of the DPPH and ABTS radicals. Total phenolic content was evaluated by Folin-Ciocalteau. Cell viability was determined by MTT assay at different concentrations (62.5; 125; 250 and 500 μg·mL<sup>-1</sup>) of EAF and HMF after 24, 48 and 72 h. Ectonucleotidase activities were evaluated by colorimetric methods after 48 h EAF or HMF treatment.</p> <p><strong>Results:</strong> The highest AA was observed for CE (76%), followed by EAF (46%) and HMF (23%). Phenolic content followed the same pattern. After 48 h, EAF increased A7r5 vascular cells viability by 40%, 40%, 62% and 25% at distinct concentrations, respectively; while HMF augmented it by 50% (500 μg·mL<sup>−1</sup>). Finally, after 48 h EAF (500 μg·mL<sup>−1</sup>) decreased about 50% of ATP and ADP metabolism while HMF inhibited 56 and 59% the hydrolysis of NPP substrate (at 125 and 250 μg·mL<sup>−1</sup>).</p> <p><strong>Conclusion:</strong> Study confirmed the high AA of <em>S. brasiliensis</em>, which influences vascular cells proliferation and purines metabolism, pointing to potential cellular pathways that may support the popular use of this plant.</p> 2020-12-31T00:00:00+00:00 ##submission.copyrightStatement## Phytochemical Investigation and Anti-Microbial Activity of Clausena anisata on Cariogenic and Periodontopathic Bacteria 2021-01-20T11:33:32+00:00 Ashu Michael Agbor Kuete Pierre Fotsing Suzy Hermine Dountio Ntungwen Charles Fokunang Sudeshni Naidoo <p><em>Clausen aanisata </em>is used in African traditional medicine to treat a variety of infectious and parasitic diseases. Infectious diseases are a major public health problem especially in developing countries where resistance to commonly used antibiotics is growing exponentially. The objective of this study was to evaluate the antibacterial activity of <em>Clausena anisata </em>on cariogenic and periodonthopatic bacteria. This was a laboratory experimental study using macerated hydroethanolic extracts of the stem and leaves of <em>Clausena anisata </em>in a water-ethanol mixture (30:70) for 48 hours. Phytochemical screening revealed flavonoids, tannins, alkaloids, coumarins, phenols, triterpenes and saponosides, these being more abundant in the leaves. The antibacterial activity of the hydroethanolic extracts was evaluated against different clinically isolated strains of three Gram negative <em>(Aggregatibacter actinomycetemcomitan, Fusobacterium nucleatum and Prevotella intermedia) </em>and two Gram positive (Streptococcus mutans and Latobacillusspp) bacteria. Polyphenols found more in the leaves extract was estimated at 62.8 mgTAE/g against 36.8 mgTAE/g in the stem bark extract. The study revealed that the leaves extract had bactericidal and bacteriostatic activity on isolated strains of <em>Aggregatibacter actinomycetemcomitan </em>and <em>Fusobacterium nucleatum </em>being the most susceptible with MICs of 50 mg/ml whereas the stem bark showed bacteriostatic activity on all isolated strains with <em>Aggregatibacter actinomycetemcomitan </em>being the most susceptible with MIC of 50 mg/ml. The antimicrobial action of the plant could be exploited in formulation of mouth washes and other medications in the management of oral infections like periodontitis and tooth dacay.</p> 2020-12-31T00:00:00+00:00 ##submission.copyrightStatement##