Role of Gabanergic and, or Serotonergic in the Anti-seizure Activity of Methanol Extract of Crinum jagus (J. Thomps) in Experimental Mice
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O. K. Wakeel
- R. A. Adebayo-Moggaji
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D. O. Wakeel
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O. O. Abiodun
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O. T. Kolawole
- A. Y. Onaolapo
- O. J. Onaolapo
- H. G. Subbair
European Journal of Medicinal Plants,
Page 36-47
DOI:
10.9734/ejmp/2022/v33i430461
Abstract
Objective: Crinum jagus is used in traditional African medicine in the treatment of epilepsy, pain and inflammations.
Methods: Anticonvulsant activity was investigated using picrotoxin (PCT) and strychnine (STR) tests in experimental paradigm.
Results: Crinum jagus leaf methanol extract (200, 400, and 800 mg kg−1) potentiated hexobarbitone-induced sleeping time and significantly (p<0.05) shortened the duration of convulsions in PCT-induced seizures. Delay in the onset of convulsions in PCT-induced seizure were very significant (p<0.05). Reduction in the frequency of seizures was also significant (p<0.05) in the test. Diazepam (5 mg kg−1) was used as reference anticonvulsant drugs in the experimental models. While, CJB failed to protect the animals against picrotocin-induced convulsion. Neither the extract of CJL nor CJB confer significant effect on STR-induced convulsion. Flumazenil (GABA receptor antagonist) and cyproheptadine (5-HT2 receptor antagonist) blocked the effect of CJL extract in the PCT tests significantly suggesting that Crinum jagus may be acting by enhancing the effects of the GABAergic and serotonergic systems.
Conclusion: The data obtained suggest that methanol leaf extract of C. jagus possessed significant anticonvulsant effect, thereby confirming the traditional uses of C. jagus in the treatment of epilepsies; mechanisms of which may involve interaction with GABAergic and serotonergic pathway.
Keywords:
- Crinum jagus
- hexobarbitone
- picrotoxin
- strychnine
- flumazenil
- serotonin
How to Cite
Wakeel, O. K., Adebayo-Moggaji, R. A., Wakeel, D. O., Abiodun, O. O., Kolawole, O. T., Onaolapo, A. Y., Onaolapo, O. J., & Subbair, H. G. (2022). Role of Gabanergic and, or Serotonergic in the Anti-seizure Activity of Methanol Extract of Crinum jagus (J. Thomps) in Experimental Mice. European Journal of Medicinal Plants, 33(4), 36-47. https://doi.org/10.9734/ejmp/2022/v33i430461
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Chu QP, Wang LE, Cui XY, Fu HZ, Lin ZB, Lin SQ, Zhang YH. Extract of Ganoderma lucidum potentiates pentobarbital-induced sleep via a GABAergic mechanism. Pharmacology Biochemistry and Behaviour. 2007;86:693-698.
Linck VM, da Silva AL, Figueiro M, Piat AL, Herrmann AP, Birck DF, Caramao EB, Nunes DS, Moreno PRH, Elisabetsky E. Inhaled linalool-induced sedation in mice. Phytomedicine. 2009;16: 303-307.
Dehar N, Walia R, Ratol,S. Potentiation of thiopentone sodium induced hypnosis by Berberis aristata in rodents. Asian Journal of Pharmacy and Clinical Research. 2012;5:131-133.
Nyeem MAB, Alam MA, Awal MA, Mostafa M, Uddin SJ, Islam N, Rouf R. CNS depressant effect of the crude ethanolic extract of the flowering tops of Rosa damascene. Iranian Journal of Pharmacology and Therapeutics. 2006;5: 171-174.
Ayoka AO, Akomolafe RO, Iwalewa EO, Akanmu MA, Ukponmwan EO. Sedative, the antiepileptic, and antipsychotic effect of Spondial mombin in mice and rats. Journal of Ethnopharmacology. 2006;103: 166–175.
Huang P, Xiao A, Zhou M, Zhu Z, Lin S, Zhang B. Heritable gene targeting in zebrafish using customized TALENs. Nature Biotechnology. 2012;29: 699–700.
Gale K. GABA and epilepsy: basic concepts from preclinical research. Epelepsia. 1992;33(5):3-12.
ISSN 0013-9580.
Ahmadiani A, Mandgary A, Sayyah M. Anticonvulsant Effect of Flutamide on seizures induced by pentylene tetrazole, the involvement of benzodiazepine receptors. Epilepsia. 2003;44(5):629-625.
Smith M, Wilcox, K.S.; White, H.S. Discovery of antiepileptic drugs. Neurotherapeutics. 2007;4:12-17.
Fabricant DS, Famswort NR. The value of plant used in traditional medicine for drug discovery. Environmental Health Journal. 2001;109:69-75.
Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sciences. 2005;78:431.
Olorode O. Taxonomy of West African Flowering Plants. Longman Publishing Company, London. 1984:121.
Ogunkunle ATJ, Olopade OR. Studies on the Asthma Coughs Plant Crinum jagus L. (Amaryllidaceae) in Nigeria. African Journal of Plant Science. 2011;5(2): 108-114.
Omobuwajo OR, Alade GO, Sowemimo A. Indigenous Knowledge and Practices of Women Herb Sellers of Southwestern Nigeria. Indian Journal of Traditional Knowledge. 2008;7(3):505-510.
Odugbemi TA. Textbook of Medicinal plants from Nigeria. 2008:Chapter II:99
Houghton PJ, Agbedahunsi JM, Adegbulugbe A. Choline esteraee inhibitory properties of alkaloids from two Nigerian Crinum species. Phytochemistry. 2004;65:2893-2896.
Adesanya SA, Olugbade TA, Odebiyi OO, Aladesanmi JA. Antibacterial alkaloids in Crinum jagus. International Journal of Pharmacognosy. 1992;30(4):303-307.
Borokin TI, Ighere DA, Clement M, Ajiboye TO, Alowonle AA. Ethnobiological survey of traditional medicine practice for the treatment of piles and Diabetes mellitus in Oyo State. Journal of Medicinal Plants Studies. 2013;1(5):30 – 40.
Dalziel JM. The useful plants of West Tropical Africa. The Crown Agents for the Colonies, London. 1937:528-560.
Kokwaro JO. Medicinal Plants of East Africa. General printers, Kenya. 1976:230
Ode OJ, Asuzu IU. The anti-snake venom activities of the methanolic extract of the bulb of Crinum jagus (Amaryllidaceae). Toxicon. 2006;48(3):331-342
Ode OJ, Nwaehujor CO, Onakpa MM. Evaluation of antihaemorrhagic and antioxidant potentials of Crinum jagus bulb. Int J Appl Biol Pharm Tech. 2010;1:1330–1336.
Shorinwa OA, Ebong OO, Obianime AW, Siminialayi IM. The effect of acetone extracts of Crinum jagus bulbs on the histology of the kidney, liver and testis of albino rats. Peak J Med Plant Res. 2013;2:38–44
Thi Ngoc Tram N, Titorenkova TV, St Bankova V, Handjieva NV, Popov SS. Review Crinum L. (Amaryllidaceae). Fitoterapia. 2002;73(3):183-208.
Nwaehujor CO, Nwinyi FC, Ode JO. Liver protective activity of the methanol extract of Crinum jargus bulb against acetaminophen-induced hepatic damage in rats. Asian J Biochem. 2012;7:182–193.
Goncalves RS, Battistin A, Pauletti G, Rota L, Serafini LA. Antioxidant properties of essential oils from Mentha species evidenced by electrochemical methods. Rev Bras Pl Med Botucatu. 2009;11:372–382.
Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med. 2012;16(12):221-225.
Ekren, S.; Yerlikaya, O.; Tokul, H.E.; Akpınar, A.; Açu, M. Chemical composition, antimicrobial activity and antioxidant capacity of some medicinal and aromatic plant extracts. Afr J Microbiol Res. 2013;7:383–388
Lorke, D. A new Approach to Acute Toxicity Testing. Archives of Toxicology. 1983;54:275-287.
Desi I, Nagymajtenyi L, Schulz H, Behavioral and neurotoxicological changes caused by cadmium treatment of rats during development. J Appl Toxicol. 1998;18(1):63–70.
Rakhshandah H, Shakeri MT, Ghasemzadeh MR. Comparative hypnotic effect of Rosa damascene fractions and diazepam in mice. Iranian Journal of Pharmaceutical Research. 2007;6:193-197.
Divya-Vohora SN, Pal KK, Pillai O. Protection from phenytoin-induced cognitive deficit by Bacopa monniera, a reputed Indian nootropic plant. Journal Ethno Pharmacology. 2000;71:383– 390.
Elisha E, Maliki SJ, Abraham BK. Effect of Jasmium officinale flower on the central nervous system of mouse. International Journal of Crude Drug Research. 1988;26:221-222.
Dilnawaz P, Shiriskumar A. Investigation of Anticonvulsant activity of Vanda rusburghii. Journal of Pharmacognosy and Phytochemistry, 2014;2:95-99.
Jiang G, Huang XJ, Chen J, Lin QS. Comparison of the sedative and hypnotic effects of flavonoids, saponins, and polysaccharides extracted from Semen Ziziphus jujube. Natural Product Research. 2007;21:310-320.
Handa SS. Extraction technology from medicinal and aromatic plant. Trieste, Italy International Center for Sciences and Technology. 2008:1-143.
Ghani A. Medicinal plants of Bangladesh with chemical constituents and uses. Dhaka, Bangladesh, Asiatic Society of Bangladesh; 2003 .
Biesaga M. “Influence of extraction methods on stability of flavonoidz”. Journal of Chromatography A. 2011;1218:2505-2512.
Datta S. Cellular and chemical neuroscience of mammalian sleep. Sleep Medicine. 2010;11:431-440.
Gottesmann C. GABA mechanisms and sleep. Neuroscience. 2002;111:231- 239.
Chu QP, Wang LE, Cui XY, Fu HZ, Lin ZB, Lin SQ, Zhang YH. Extract of Ganoderma lucidum potentiates pentobarbital-induced sleep via a GABAergic mechanism. Pharmacology Biochemistry and Behaviour. 2007;86:693-698.
Linck VM, da Silva AL, Figueiro M, Piat AL, Herrmann AP, Birck DF, Caramao EB, Nunes DS, Moreno PRH, Elisabetsky E. Inhaled linalool-induced sedation in mice. Phytomedicine. 2009;16: 303-307.
Dehar N, Walia R, Ratol,S. Potentiation of thiopentone sodium induced hypnosis by Berberis aristata in rodents. Asian Journal of Pharmacy and Clinical Research. 2012;5:131-133.
Nyeem MAB, Alam MA, Awal MA, Mostafa M, Uddin SJ, Islam N, Rouf R. CNS depressant effect of the crude ethanolic extract of the flowering tops of Rosa damascene. Iranian Journal of Pharmacology and Therapeutics. 2006;5: 171-174.
Ayoka AO, Akomolafe RO, Iwalewa EO, Akanmu MA, Ukponmwan EO. Sedative, the antiepileptic, and antipsychotic effect of Spondial mombin in mice and rats. Journal of Ethnopharmacology. 2006;103: 166–175.
Huang P, Xiao A, Zhou M, Zhu Z, Lin S, Zhang B. Heritable gene targeting in zebrafish using customized TALENs. Nature Biotechnology. 2012;29: 699–700.
Gale K. GABA and epilepsy: basic concepts from preclinical research. Epelepsia. 1992;33(5):3-12.
ISSN 0013-9580.
Ahmadiani A, Mandgary A, Sayyah M. Anticonvulsant Effect of Flutamide on seizures induced by pentylene tetrazole, the involvement of benzodiazepine receptors. Epilepsia. 2003;44(5):629-625.
Smith M, Wilcox, K.S.; White, H.S. Discovery of antiepileptic drugs. Neurotherapeutics. 2007;4:12-17.
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