Effects of Hydroethanolic Extract of Adenia lobata (Jacq.) Engl. (Passifloraceae) on Nociception and Subsequent Anxiety-like Behavior: Both Anti-inflammatory and Antioxidant Approaches
European Journal of Medicinal Plants,
Aims: Adenia lobata (Jacq.) Engl. (Passifloraceae) is widely used in Ivorian traditional pharmacopeia to heal various chronic diseases, relieve headache and pain of gingiva inflammation, and facilitate labor. Here, we investigated the effects of hydroethanolic extract Adenia lobata (HEAL) on nociceptive pain and subsequent anxiety-like behavior.
Materials and Methods: We used several experimental pain tests as the writhing, formalin and hot plate to evaluate both antinociceptive and anti-inflammatory actions of the extract. Anxiety related to nociception was tested with open field and elevated plus maze tests. Then, mice were sacrificed for assessing some oxidative stress markers.
Results: The extract of 30 mg/kg, p.o. reduced in the similar manner as reference peripheral drug salcylicacetic acid (ASA, 200 mg/kg, i.p.) the number of writhings induced by acid acetic. In both neurogenic and inflammatory phases of formalin test, the extract demonstrated an effective antinociceptive activity than ASA, but comparable to central analgesic tramadol (50 mg/kg, i.p). However, Adenia lobata reduced lesser thermal-induced pain than tramadol in hot plate test, but significantly compared to ASA. Furthermore, HEAL altered anxiety-like behavior in each case of the pain condition studied. Also, the extract showed the highest antioxidant activity by reduction oxide nitric (NO) and malondialdehyde (MDA), and increase non protein thiol (NP-SH) levels.
Conclusion: In conclusion, HEAL possesses antinociceptive and anti-inflammatory actions on peripheral and central mechanisms of pain. The phytochemicals components of the extract as alkaloids and flavonoids suggest to interact with the opioid system and combat the oxidative stress, respectively. Our findings provide scientific basis for the use of Adenia lobata in traditional medicine against pain and related diseases.
- Adenia lobata
- antinociceptive activity
- anxiety-like behavior
How to Cite
Miller LR, Cano A. Comorbid chronic pain and depression: who is at risk?” Journal of Pain. 2009;10(6):619–627.
Asmundson GJG, Katz J. “Understanding the co- occurrence of anxiety disorders and chronic pain: state-of-the- art,” Depression and Anxiety. 2009;26(10): 888–901,R.
Gatchel J, Peng Y B, Peters ML, Fuchs PN, Turk DC. “The biopsychosocial approach to chronicpain: scientific advances and future directions,” Psychological Bulletin. 2007;133(4):581–624.
Cury Y, Picolo G, Gutierrez VP, Ferreira, SH. Pain and analgesia: The dual eﬀect of nitric oxide in the nociceptive system. Nitric Oxide. 2011;25:243–254.
Guo TZ, Wei T, Huang TT, Kingery WS, Clark JD. Oxidative stress contributes to fracture/cast-induced inflammation and pain in a rat model of complex regional pain syndrome. J Pain. 2018;19(10): 1147–1156.
Hendrix J, Nijs J, Ickmans K, Godderis L, Ghosh M, Polli A. The Interplay between Oxidative Stress, Exercise, and Pain in Health and Disease: Potential Role of Autonomic Regulation and Epigenetic Mechanisms. Antioxidants. 2020;9(11): 1166.
Katzung BG. Basic and Clinical Pharmacology, 8th ed. Appleton and Lange, USA. 2001;523–525,602–605.
N’guessan K. Contribution to the ethnobotanical survey in Krobou country. Thesis of 3rd cycle doctorate, National University of Côte d'Ivoire. 1995;557.
Agoreyo BO, Aweh PAP, Agoreyo FO. Effects of different storage conditions on the Ascorbic acid content of plantain (Musa paradisiaca L.). Bioscience Research Communications. 2012;15:239-244.
Farouk L, Laroubi A, Aboufatima R, Benharref A, Chait A. Evaluation of the analgesic effect of alkaloid extract of Peganum harmala L. Possible mechanisms involved ournal of Ethnopharmacology. 2008;115: 449–454.
Prut L. Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: A review. 2003; 463(1-3):3-3.
Dawson GR, Tricklebank M.D. Use of the elevated plus-maze in the search for novel anxiolytic agents. Trends Pharmacol. Sci. 1995;16:33.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82:70–77.
Touil-Boukoffa C, Bauvois B, Sancéau J, Hamrioui B, Wietzerbin J. Production of nitric oxide (NO) in human hydatidosis: relationship between nitrite production and interferon-gamma levels. Biochimie. 1998;80 (8-9):739-744
Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta. 1978;90:37–43.
Arif H, Aggarwal S. Salicylic Acid (Aspirin). In: StatPearls. Treasure Island (FL): Stat Pearls Publishing; 2020.
Ridtitid W, Sae-wong C, Reanmongkol W, Wongnawa M. Antinociceptive activity of the methanolic extract of Kaempferia galanga Linn. in experimental animals. Journal of Ethnopharmacology. 2008; 118(2):225–230.
N’guessan K, Zirihi NG. Boraud N. Étude ethnopharmacologique des plantes utilisées pour faciliter l’accouchement, en pays Abbey et Krobou, au Sud de la Cote-d’Ivoire. International Journal of Biological and Chemical Sciences. 2010;4(4):1004-1016.
Okokon JE, Davis K, Nwidu LL. Anti-inflammatory and antinociceptive activities of Solenostemon monostachyus aerial part extract in mice. Avicenna J Phytomed. 2016;6(3): 284-294.
Fowa AB, Fodouop SPC, Fukunaga CN, Djoueudam FG, Famen LCN, Ongbayokolak NS, et al. Antityphoid and antioxidant activities of hydroethanolic leaf extract of Adenia lobata Jacq. (Passifloraceae) on Salmonella typhi infected wistar rats. Journal of Medicinal Plants Studies. 2019;7(1): 13-22.
Carlo DG, Mascolo N, Izzo AA, Capasso F. Flavonoids, old and new aspects of a class of natural therapeutic drugs. Life Sci. 1999;65:337–353.
Amin AR, Attur MG, Pillinger M, Abramson SB. The pleiotropic functions of aspirin: mechanisms of action. Cell Mol Life Sci. 1999;56:305–12.
Le Bars D, Gozariu M, Cadden SW. Animal models of nociception. Pharmacological Review. 2001;53:597–652.
Yamasaki H, Funai Y, Funao T, Mori T, Nishikawa K. Effects of Tramadol on Substantia Gelatinosa Neurons in the Rat Spinal Cord: An In Vivo Patch-Clamp Analysis. PLoS ONE. 2015;10(5): e0125147.
Raffa RB, Friderichs E, Reimann W, Shank RP, Codd EE, Vaught JL. Opioid and nonopioid components independently contribute to the mechanism of action of tramadol, an 'atypical' opioid analgesic. The Journal of pharmacology and experimental therapeutics. 1992; 260(1):275 – 85.
Shibata M, Okhubo T, Takahashi H, Inoki R. Modiﬁed formalin test: characteristics biphasic pain response. Pain.1989;38: 346–352.
Gao KX, Zhao Q, Wang GR, Yu L, Wu JY, Zhao X. Isorhynchophylline exerts antinociceptive effects on behavioral hyperalgesia and allodynia in a mouse model of neuropathic pain: evidence of a 5-HT(1A) receptor-mediated mechanism. Front Pharmacol. 2020; 11:318.
Lara CO, Murath P, Muñoz B, Marileo A M, Martín LS, San Martín VP. et al. Functional modulation of glycine receptors by the alkaloid gelsemine. British Journal of Pharmacology, 2016;173(14): 2263–2277.
McCormick SJ, Tunnicliff G. Inhibitors of synaptosomal gamma- hydroxybutyrate transport. Pharmacology. 1998;57:124–131.
Abstract View: 61 times
PDF Download: 18 times