Antioxidant Activities of the Aqueous Extracts of Pedalium murex D. Royen EX L. Fruit and Leafy Stem
European Journal of Medicinal Plants,
Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit.
Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay.
Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract while they were equal to 26.26± 0.54 µg/mg GAE; 47.88± 2.39 µg/mg RUE; 7.94± 0.31 µg/mg TAE for the fruit. The leafy stem extract exhibited a more pronounced DPPH scavenging activity, reducing power, hydrogen peroxide scavenging activity and lipid peroxidation activity than the fruit extract.
Conclusion: The antioxidant activity of the leafy stem aqueous extract was significantly more potent than that of the fruit extract. Further studies will find out the extracts pharmacological efficacy and innocuity.
- Oxidative stress
- Pedalium murex
- phenolics content
How to Cite
Migdal C, Serres M. Espèces réactives de l ’ oxygène et stress oxydant. médecine/sciences. 2011;27:405-412.
Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, et al. Oxidative Stress , Prooxidants , and Antioxidants : The Interplay. 2014;2014.
Vander Borght M, Wyns C. Fertility and infertility: Definition and epidemiology. Clin Biochem. 2018;62:2-10.
Chehab M, Madala A, Trussell JC. On-label and off-label drugs used in the treatment of male infertility. Fertil Steril. 2015;103(3):595-604.
Jung JH, Seo JT. Empirical medical therapy in idiopathic male infertility: Promise or panacea? Clin Exp Reprod Med. 2014;41(3):108-114.
Smits RM, Mackenzie-Proctor R, Yazdani A, Stankiewicz MT, Jordan V, Showell MG. Antioxidants for male subfertility. Cochrane Database Syst Rev. 2019;2019(3):233.
Ekor M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014;4(177):1-10.
Aouachria S, Boumerfeg S, Benslama A, Benbacha F. Acute , sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardia picroide crude extract. J Ethnopharmacol. 2017;208:105-116.
Gutteridge JMC, Halliwell B. Biochemical and Biophysical Research Communications Antioxidants : Molecules , medicines , and myths. Biochem Biophys Res Commun. 2010;393(4):561-564.
Gulumian M, Yahaya ES, Steenkamp V. African Herbal Remedies with Antioxidant Activity : A Potential Resource Base for Wound Treatment. Evidence-Based Complement Altern Med. 2018;2018.
Patel DK, Laloo D, Kumar R, Hemalatha S. Pedalium murex Linn.: An overview of its phytopharmacological aspects. Asian Pac J Trop Med. 2011;4(9):748-755.
Edoun FLE, Tchuente BRT, Dibacto REK, Mouafo HT, Tchuenchieu ADK, Ndzana AAC, et al. Phytochemical Screening and Antioxidant Potential of Aqueous Extracts of Millettia laurenti , Lophira alata and Milicia excelsa, Commonly Used in the Cameroonian Pharmacopoeia. European J Med Plants. 2020;31(11):11-23.
Osagie-eweka SDE, Orhue NJ, Ekhaguosa DO. Comparative Phytochemical Analyses and in-vitro Antioxidant Activity of Aqueous and Ethanol Extracts of Simarouba glauca ( Paradise Tree ). European J Med Plants. 2016;13(3):1-11.
Price ML, Butler LG. Rapid Visual Estimation and Spectrophotometric Determination of Tannin Content of Sorghum Grain. J Agric Food Chem 1977;25(6):1268-1273.
Akomolafe SF, Oboh G, Akindahunsi AA, Afolayan AJ. Tetracarpidium conophorum ameliorates oxidative reproductive toxicity induced by ethanol in male rats. BMC Complement Altern Med. 2015;15(1):1-9.
Oyaizu M. Antioxidative Activities of Products of Browning Reaction Prepared from Glucosamine. Japanese J Nutr Diet. 1986;4(6):307-315.
Mukhopadhyay D, Dasgupta P, Sinha Roy D, Palchoudhuri S, Chatterjee I, Ali S, et al. A Sensitive In vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay using 1,10-Phenanthroline. Free Radicals Antioxidants. 2016;6(1):124-132.
Sadiq MB, Tharaphan P, Chotivanich K, Tarning J, Anal AK. In vitro antioxidant and antimalarial activities of leaves, pods and bark extracts of Acacia nilotica (L.) Del. BMC Complement Altern Med. 2017 ;17(1):1-8.
Yu M, Gouvinhas I, Rocha J, Barros AIRNA. Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources. Sci Rep. 2021;11:1-14.
Sadeer NB, Montesano D, Albrizio S, Zengin G, Mahomoodally MF. The Versatility of Antioxidant Assays in Food Science and Safety — Chemistry , Applications , Strengths , and Limitations. Antioxidants. 2020;9(709):1-39.
Thakkar J, Solanki A, Thakkar M, Solanki H, Patel N. In Vitro Antioxidant Activity of Aqueous Fruit Extract of Pedalium murex. Int J Preclin Pharm Res. 2011;2(1):26‑29.
Swaminathan G, Jupudi S, Sathish R, Roshan NS, Rajagopal K. Phytochemical and pharmacological assessment of leaf extract of Pedalium murex linn herb. Int J Res Pharm Sci. 2020;11(4):6557-6563.
Kobus-Cisowska J, Szczepaniak O, Szymanowska-Powałowska D, Piechocka J, Szulc P, Dziedziński M. Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition. Ciência Rural. 2020;50(1): 1-12.
Subramanian R, Subbramaniyan P, Raj V. Antioxidant activity of the stem bark of Shorea roxburghii and its silver reducing power. Springerplus. 2013;2(28):1-11.
Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005;53(10):4290-4302.
Siqueira P, Bolini H, Macedo G. Polyphenols and antioxidant properties in forced and naturally aged Brazilian beer. J Brew Distill 2011;2(May):45-50.
Patel DK, Kumar R, Prasad SK, Hemalatha S. Pedalium murex Linn (Pedaliaceae) fruits: A comparative antioxidant activity of its different fractions. Asian Pac J Trop Biomed. 2011;1(5): 395-400.
Bajpai VK, Kim Y, Baek K-H. Phenolic Content , Lipid Peroxidation Inhibition and Antioxidant Potential of Leaf Essential Oil of Ginkgo biloba in Various Scavenging Models. Natl Acad Sci Lett. 2017;:5-9.
Zhang W, Chen H, Wang Z. Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai. J Food Sci Technol. 2013;50:1122-1129.
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants. 2019;8(96): 1-12.
Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011;82(4): 513-523.
Gharagozloo P, Aitken RJ. The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Hum Reprod. 2011;26(7): 1628-1640.
Wintola O, Afolayan A. Phytochemical constituents and antioxidant activities of the whole leaf extract of Aloe ferox Mill . Pharmacogn Mag 2011;7(28).
Priya P, Rao A. Evaluation of in vitro free radical scavenging activity of Crinum asiatica and Pedalium murex leaf extracts. IOSR J Pharm. 2016;6(2): 37-43.
Iqbal E, Abu K, Lim LBL. Phytochemical screening , total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus ( Airy Shaw ) from Brunei Darussalam. J King Saud Univ - Sci. 2015;27(3):224-232.
Rice-Evans C, Miller JN, Paganga G. Antioxidant properties of phenolic. Trends Plant Sci. 1997;2(4):152-159.
Koleckar V, Kubikova K, Rehakova K, Jun D, Jahodar L, Opletal L. Condensed and Hydrolysable Tannins as Antioxidants Influencing the Health. Mini-Reviews Med Chem. 2008;8(5):436-447.
Abstract View: 123 times
PDF Download: 46 times