Isolation, Characterization and Biological Activities of Stigmasterol from Leaf Part of Crescentia alata Kunth (Bignoniaceae)
European Journal of Medicinal Plants,
The present investigation deals with the extraction, separation, isolation, identification, characterization and biological evaluation of the stigmasterol from methanolic extract on leaves of C. alata Kunth using bio-guided fractionation and spectral analytical methods. The biological activities investigated were antimicrobial and cytotoxicity. Leaf crude extract of C. alata obtained from 80% methanol was successively extracted with hexane, chloroform, ethyl acetate and n-butanol. Ethyl acetate fraction afforded a bioactive compound by bioassay-guided fractionation. The characterization of isolates was done by biochemical and spectral methods. The active fraction obtained and isolated compound were tested for their antimicrobial activities. The cytotoxicity of the isolated compound on Hela cell line, estimated with the MTT assay. The ethyl acetate fraction has exhibited highest effective antimicrobial activities and the fraction afforded a compound stigmasterol. The compound isolated stigmasterol from leaf of C. alata showed strongest antimicrobial effect against all microbial strains were tested with minimum inhibitory concentration values ranging from 1.95 to 125 μg/mL. The cytotoxicity studies indicated that the isolated stigmasterol possesses much potential against Hela (mammalian cancer) cell line. Overall, the stigmasterol compound was the most dynamic as far as the antibacterial and antifungal potential of the leaves of C. alata confirm the conventional utilization of this plant in treating different respiratory sufferings and its related manifestations. The properties of the bioactive phytochemical compound stigmasteol recommend that the powerful and wide range of antimicrobial and anticancer operators and may fill in as the lead compound in the advancement of novel restorative medications.
- Crescentia alata
- Bio-guided isolation
- Spectral analysis
- Antimicrobial activity
- Cytotoxicity studies
- Pure compound
- Medicinal plant
- Traditional medicines
How to Cite
O'Neill J. Tackling drug-resistant infections globally: final report and recommendations; 2016.
Tittikpina NK. Contribution à L’évaluation des Propriétés Anti-Microbiennes de: Pterocarpus Erinaceus Poir (Faboïdeae), Daniellia Oliveri (Rolfe) Hutch. et Dalz (Caesalpinoïdeae) et Anchomanes Difformis (Blume) Engler (Araceae), Utilisées en Médecine Traditionnelle dans la Préfecture de Tchamba (TOGO). Doc de Pharmacie, Univ. Lomé. 2012;87.
World Health Organization, & UNICEF. Global report for research on infectious diseases of poverty; 2012.
Von Poser GL, Schripsema J, Henriques AT, Jensen SR. The distribution of iridoids in Bignoniaceae. Biochemical Systematics and Ecology. 2000;28(4):351-366.
Cano L. Flora medicinal indígena de Méxicotreintay cinco monografías del atlas de las plantas de la medicina tradicional mexicana (No. C/306.4672 B5/5); 1994.
Gilman EF, Watson DG. Camellia oleifera (Tea-oil camellia). United States Forest Service Fact Sheet, ST-116; 1993.
Valladares-Cisneros MG, Rios-Gomez MY, Aldana-Llanos L, Valdes-Estrada ME, Ochoa MG. Biological activity of Crescentia alata (Lamiales: Bignoniaceae) fractions on larvae of Spodoptera frugiperda (Lepidoptera: Noctuidae). Florida Entomologist. 2014;97(2):770-777.
Arenas FS. Etnobotánica y usos potenciales del Cirián (Crescentia alata, HBK) en el estado de Morelos. Polibotánica. 2004;(18):13-31.
Zaveri M, Jain S. Gastroprotective effects of root bark of Oroxylum indicum, vent. Journal of Natural Remedies. 2007;7(2):269.
Khandhar M, Shah M, Santani D, Jain S. Antiulcer Activity of the root bark of Oroxylum indicum. against experimental gastric ulcers. Pharmaceutical biology. 2006;44(5):363-370.
Silva TMS, Da Silva TG, Martins RM, Maia GLA, Cabral AGS, Camara CA, et al. Molluscicidal activities of six species of Bignoniaceae from north–eastern Brazil, as measured against Biomphalaria glabrata under laboratory conditions. Annals of Tropical Medicine & Parasitology. 2007;101(4):359-365.
Kaushik R, Saini P. Larvicidal activity of leaf extract of Millingtonia hortensis (Family: Bignoniaceae) against Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. Journal of vector borne diseases. 2008;45(1):66.
Pillay P, Maharaj VJ, Smith PJ. Investigating South African plants as a source of new antimalarial drugs. Journal of Ethnopharmacology. 2008;119(3):438-454.
Kaur N, Chaudhary J, Jain A, Kishore L. Stigmasterol: A comprehensive review. International Journal of Pharma- ceutical Sciences and Research. 2011;2(9):2259.
Rao AV, Janezic SA. The role of dietary phytosterols in colon carcinogenesis; 1992.
Buwa LV, Afolayan AJ. Antimicrobial activity of some medicinal plants used for the treatment of tuberculosis in the Eastern Cape Province, South Africa. African Journal of Biotechnology. 2009;8(23).
Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta medica. 1998;64(08):711-713.
Aremu AO, Fawole OA, Chukwujekwu JC, Light ME, Finnie JF, Van Staden J. In vitro antimicrobial, anthelmintic and cyclooxygenase-inhibitory activities and phytochemical analysis of Leucosidea sericea. Journal of ethnopharmacology. 2010;131(1):22-27.
Vasanthakumar V, Saranya A, Raja A, Prakash S, Anbarasu V, Priya P, Raj V. The synthesis, characterization, removal of toxic metal ions and in vitro biological applications of a sulfanilamide–salicylic acid–formaldehyde terpolymer. RSC advances. 2016;6(60):54904-54917.
Domínguez XA, Sanchez H, Merijanian BA, Rojas-MP. Stigmasterol, friedool- eanan-3beta-ol and Baccharis oxide from Baccharis salicifolia. Phytochemistry; 1972.
Ahmed Y, Sohrab MH, Al-Reza SM, Tareq FS, Hasan CM, Sattar M. A. Antimicrobial and cytotoxic constituents from leaves of Sapium baccatum. Food and Chemical Toxicology. 2010; 48(2):549-552.
Jain PS, Bari SB. Isolation of lupeol, stigmasterol and campesterol from petroleum ether extract of woody stem of Wrightia tinctoria. Asian Journal of Plant Sciences. 2010;9(3):163.
Chaturvedula VSP, Prakash I. Isolation of Stigmasterol and?-Sitosterol from the dichloromethane extract of Rubus suavissimus. International Current Pharmaceutical Journal. 2012;1(9):239-242.
Jamaluddin F, Mohamed S, Lajis MN. Hypoglycaemic effect of Parkia speciosa seeds due to the synergistic action of β-sitosterol and stigmasterol. Food Chemistry. 1994;49(4):339-345.
Jain SC, Singh B, Jain R. Antimicrobial activity of triterpenoids from Heliotropium ellipticum. Fitoterapia. 2001;72(6):666-668.
Panda S, Jafri M, Kar A, Meheta BK. Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated from Butea monosperma. Fitoterapia. 2009;80(2):123-126.
Habib MR, Nikkon F, Rahman M, Haque ME, Karim MR. Isolation of stigmasterol and beta-sitosterol from methanolic extract of root bark of Calotropis gigantea (Linn). Pakistan journal of biological sciences: PJBS. 2007;10(22):4174-4176.
York T, Van Vuuren SF, De Wet H. An antimicrobial evaluation of plants used for the treatment of respiratory infections in rural Maputaland, KwaZulu-Natal, South Africa. Journal of Ethnopharmacology. 2012;144(1):118-127.
Madikizela B, Aderogba MA, Finnie JF, Van Staden J. Isolation and characterization of antimicrobial compounds from Terminalia phanerop- hlebia Engl. & Diels leaf extracts. Journal of ethnopharmacology. 2014;156:228-234.
Abstract View: 187 times
PDF Download: 175 times