Cardioprotective Activities of Pterocarpus mildbraedii Leaves on Isoproterenol-induced Myocardial Infarction in Rats

Main Article Content

A. O. Fajobi
B. O. Emma- Okon
O. O. Oyedapo
C. A. Dare
A. O. Akinlalu
I. J. Komolafe
M. Ogunsusi

Abstract

The study evaluated the effects of Pterocarpus mildbraedii leaf extracts on myocardial infarction induced by isoproterenol (ISO) in Wistar albino rats with a view to ascertain the value of its use in the management of heart-related diseases. Fresh plant leaves were collected, identified, extracted, fractionated and the aqueous layer partitioned with ethyl acetate. GC-MS was carried out on the ethyl acetate fraction (EAF) and unknown compounds were identified by comparing measured mass spectral data with those in NIST 14 Mass Spectral Library. Twenty-five adult rats were divided into five groups of 5 rats each. Groups I &II were the control groups. Rats in groups III-V were pretreated with 50 mg/kg and 100 mg/kg of EAF and 1.8 mg/kg of propranolol respectively for 21 days. Myocardial infarction was then induced in all the rats (except those in Group I) with the intraperitoneal injection of ISO (85 mg/kg) for 2 days. Afterwards, the rats were sacrificed and blood samples, heart homogenate and samples for histological studies were aseptically collected. Activities of cardiac biomarkers, lipid profile, enzymatic and non-enzymatic antioxidants were evaluated using standard methods. GC-MS analysis showed that the most abundant components of the plant are propionic acid, 2,3-dimethylphenyl ester, catechol, octyl-β-D-glucopyranoside, phenol and n-Hexadecanoic acid. Administration of ISO caused significant elevation of the activities of cardiac biomarkers (troponin-T concentrations, creatine kinase-MB and lactate dehydrogenase) while rats pretreated with EAF had significantly lower levels of the biomarkers. Moreover, alterations in lipid profile, enzymatic and non-enzymatic antioxidants brought about by the administration of ISO were ameliorated. Histological examinations revealed lesser degree of myocardial injury in pre-treated rats.

Keywords:
Pterocarpus mildbraedii leaves, isoproterenol, myocardial infarction, GC-MS, Troponin-T, CK-MB, LDH, lipid profile

Article Details

How to Cite
Fajobi, A. O., Okon, B. O. E.-, Oyedapo, O. O., Dare, C. A., Akinlalu, A. O., Komolafe, I. J., & Ogunsusi, M. (2020). Cardioprotective Activities of Pterocarpus mildbraedii Leaves on Isoproterenol-induced Myocardial Infarction in Rats. European Journal of Medicinal Plants, 31(10), 20-37. https://doi.org/10.9734/ejmp/2020/v31i1030278
Section
Original Research Article

References

WHO. Cardiovascular disease.

Available:www.who.int/cardiovasculr_diseases/about_cvd/en/2020

(Accessed 10/02/2020)

Kuruwitaarachchige VS, Uluwaduge DI, Premakumara S, Wijayabandara J. Cardioprotective activity of Abelmoschus esculentus (Okra). Int J Food Sci Nutr. 2018;3(5):39-43.

Shier D, Butler J, Lewis R. Skeletal system and blood and cardiovascular system. In: Hole’s Human Anatomy and Physiology Thirteenth Edition McGraw-Hill, New York. 2013;201-615.

Huma S, Tariq R, Amin F, Mahmood KT. Modifiable and non-modifiable predisposing risk factors of myocardial infarction- A review. J Pharm Sci Res. 2012;4(1):1649-1653.

Thygesen K, Alpert JS, Jaffe AS, Chaitman BR, Bax JJ, Morrow DA, White HD, Mickley H, Crea F, Van de Werf F, Bucciarelli-Ducci C, Katus HA, Pinto FJ, Antman EM, Hamm CW, De Caterina R, Januzzi Jr JL, Apple FS, Garcia MAA, Underwood SR, Canty Jr JM, Lyon AR, Devereaux PJ, Zamorano JL, Lindahl B, Weintraub WS, Newby LK, Virmani R, Vranckx P, Cutlip D, Gibbons RJ, Smith SC, Atar D, Luepker RV, Robertson RM, Bonow RO, Steg PG, O’Gara PT, Fox KAA. Fourth universal definition of myocardial infarction. Eur Soc Cardiol. 2018;1-33.

Frangogiannis NG. Pathophysiology of myocardial infarction. Compl Physiol. 2015;5:1841-1875.

Afroz R, Tanvir EM, Karim N, Hossain S, Alam N, Gan SH, Khalil I. Sundarban honey confers protection against isoproterenol-induced myocardial infarction in Wistar rats. Biomed Res Int. 2016;1-10. Aticle ID: 6437641.

DOI: 10.1155/2016/6437641

Morakinyo AE, Oyedapo OO, Akinpelu BA, Fajobi AO, Fasakin WO, Komolafe IJ, Akinlalu AO. Evaluation of antioxidant, anti-inflammatory and phytochemical constituents of Aframomum melegueta aqueous leaf extract. Int J Med Plants Photon. 2018;112:888-903.

Upaganlawar A, Balaraman R. Cardioprotective effects of Lagenaria siceraria fruit juice on isoproterenol-induced myocardial infarction in wistar rats: A biochemical and histoarchitecture study. J Young Pharmacists. 2011;3(4):297-303.

Khalil I, Ahmmed I, Ahmed R, Tanvir EM, Afroz R, Paul S, Gan SH, Alam N. Amelioration of isoproterenol-induced oxidative damage in rat myocardium by Withania somnifera leaf extract. BioMed Res Int. 2015;10. Article ID: 624159.

DOI: 10.1155/2015/624159

Adegbite AV, Ezekwesili CN, Okani OC. Effects of ethanol and aqueous leaf extracts of Pterocarpus mildbraedii on the liver function and CD4 cells of the immune system in albino rats. The Biochemist. 2015;3(1):79-87.

Ucheghu RI, Iwuoha GU, Elenwoke UE, Ibe CO, Amanze KO. Identification of phytochemicals present in the leaves of Pterocarpus mildbraedii harms by GC/MS analysis. IOSR J Appl Chem. 2015;8(7):9-10.

Adeyemi AA, Dike IM. Evaluation of Pterocarpus mildbraedii Harm. performance under different growth conditions. J Sust Envir Manag. 2018;10: 46-62.

Oyedapo OO, Amos S. Further investigations into the bioactivity of root extract of Plumbago zeylanica. Phyt Res. 1997;11:62-63.

Oyedapo OO, Akinpelu BA, Akinwunmi KF, Adeyinka MO, Sipeolu FO. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. Int J Plant Physiol Biochem. 2010;2(4):46-51.

Mollina-Salinas GM, Pena-Rodriguez LM, Mata-Cardenas BD, Escalante-Erosa F, Gonzalez-Hernandez S, Torres de la Cruz VM, Matinez-Rodriduez HG, Said-Fernandez S. Flourensia cernua: Hexane extracts a very active mycobacterial fraction from an inactive leaf decoction against pansensitive and panresistant mycobaterium tuberculosis. Evid Based Complement Alternat Med. 2011;9. Article ID: 782503.

DOI: 10.1155/2011/782503

Bode SO, Oyedapo OO. Biological activities and phytoconstituents of the lower plant Platycerium anglolense, Welwex Hook. J Med Plants Res. 2011;5(8):1321-1329.

Maklund S, Maklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47:469-474.

Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972;47(2):389-394.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hofeman DG, Hoekstra WG. Selenium: Biochemical role as a component of glutathione peroxidase. Sci. 1973;179(73):283-285.

Moron MS, Depierre JW, Manneryik B. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biophys Acta. 1979;582:67-78.

Omaye ST, Turnbull JD, Sauberlich HE. Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. In: McCormick D.B. and Wright D.L. (Eds). Methods of Enzymology New York: Academic Press. 1979;3-11.

Baker H, Frank O. Tocopherol. Methods and interpretation. New York Interscience Publisher, John Wiley and Sons, Inc. Clinical Vitaminology. 1968;172-173.

Deepthi GK, Divakar TE, Murthy NB, Pavankumar D. Spectrophotometric methods for the determination of lipoic acid by using MBTH and ferric acid. Int J Eng Res Technol. 2012;1(7):1-7.

Prophet EB, Mills B, Arrington JB, Sobin LH. Laboratory methods in histotechnology. Amer J. Clin Path. 1992;14(3):25-33.

Kaur C, Kapoor HC. Antioxidants in fruits and vegetables - the Millennium’s health. Int J Food Sci Technol. 2001;36:703-725.

Ravimannan N, Nisansala A. Study on antioxidant activity in fruits and vegetables: A review. Int J Adv Res Biol Sci. 2017;4(3): 93-101.

Orishadipe AT, Okogun JI, Mishelia E. Gas chromatography-mass spectrometry analysis of the hexane extract of Calliandra portoricensis and its antimicrobial activity. Afr J Pure Appl Chem. 2010;4(7):131-134.

Haider MH, Imad HH, Omar AI. Antimicrobial activity and spectral chemical analysis of methanolic leaves extract of Adiantum capillus-veneris using GC-MS and FT-IR spectroscopy. Int J Pharmacog Phytochem Res. 2016;8(3):369-385.

Karthikeyan V, Baskaran A, Rajasekaran CS. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of ethanolic extracts of Barleria acuminata Nees. Int J Pharm Res. 2016;6(2):55-61.

Ganesh M, Mohankumar M. Extraction and identification of bioactive components in Sida cordata (Burm.f.) using gas chromatography-mass spectrometry. J. Food Sci Technol. 2017;54(10):3082-3091.

Choudhary D, Shekhawat JK, Kataria V. GC-MS analysis of bioactive phytochemicals in methanol extract of aerial part and callus of Dipterygium glaucum Decne. Pharmacogn J. 2019;11(5):1055-1063.

Mythili S, Malathi N. Diagnostic markers of acute myocardial infarction (Review). Biomed Rep. 2015;3:743-748.

Li H, Xie Y-H, Yang Q, Wang S-W, Zhang B-L, Wang J-B, Cao W, Bi L-L, Sun J-Y, Miao S, Hu J, Zhou X-X, Qiu P-C. Cardioprotective effect of Paenol and Danshensu combination on isoproterenol-induced myocardial injury in rats. PLoS ONE. 2012;7(11):1-10.

Hu X, Wang H, Lv Xinhu, Chu L, Liu Z, Wei X, Chen Q, Zhu L, Cui W. Cardioprotective effects of tannic acid on isoproterenol-induced myocardial injury in rats: Further insight into ‘French paradox’. Physioth Res. 2015;29:1295-1303.

Obouayeba AP, Meite S, Boyvin L, Yeo D, Kouakou TH, N’Guessan JD. Cardioprotective and anti-inflammatory activities of a polyphenols enriched extract of Hibiscus sabdariffa petal extracts in Wistar rats. J Pharmacog Phytochem. 2015;4(1):57-63.

Braun K, Oecki J, Westermeler J, Li Y, Klingenspor M. Non-adrenergic control of lipolysis and thermogenesis in adipose tissues. J Exp Biol. 2018;221:1-14.

Haselgrubler R, Lanzerstorfer P, Rohrl C, Stubl F, Schurr J, Schwarzinger B, Schwarzinger C, Brameshuber M, Wieser S, Winkler S, Weghuber J. Hypolipidemic effects of herbal extracts by reduction of adipocyte differentiation, intracellualar neutral lipid content, lipolysis, fatty acid exchange and lipid droplet motility. Sci Rep. 2019;9(10492):1-18.

Stockil J, Zadoorian A, Cooke KC, Deshpande V, Yau B, Herrmann G, Kebede MA, Humphrey SJ, James DE. ABHD15 regulates adipose tissue lipolysis and hepatic lipid accumulation. Mol Metabol. 2019;25:83-94.

Bertholf RL. Proteins and albumin. LabMedicine. 2014;45(1):e25-e41.

Ndem JI, Otitoju O, Akpanaiabiatu MI, Uboh FE, Uwah AF, Edet OA. Haematoprotective property of Eremomastax speciosa (Hochst.) on experimentally induced anaemic Wistar rats. Ann Biol Res. 2013;4(6):356-360.

Fajobi AO, Fasakin OW, Komolafe IJ, Akinlalu AO, Morakinyo AE, Oyedapo OO. Cardioprotective activities of Piper guineense seeds on isoproterenol-treated rats. Nig J Biochem Mole Biol. 2017;32(1): 10-22.

Komolafe K, Akinmoladun AC, Olaleye MT. Methanolic leaf extract of Parkia biglobosa protects against doxorubicin-induced cardiotoxicity in rats. Int J Appl Res Nat Prod. 2013;6(3):39-47.

Akinlalu AO, Komolafe IJ, Ogunsusi M, Oyedapo OO. Bioactivity of Platycerium angolense flavonoid fraction on biochemical parameters of acetaminophen-induced rats. J Med Plants Res. 2016;10(33):559-569.

Adekunle AS, Adelusi TI, Oyewo EB, Kamdem J-P, Akintade BB. Antihypercholesterolemic, cardioprotective and vitamins E and C sparing properties of Bryophyllum pinnatum in rabbits. Eur J Med Plants. 2016;11(3):1-13.

Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K. Oxidative stress, poroxidants and antioxidants: The interplay. BioMed Res Int. 2014;1-19. Article ID: 761264.

Skibska B, Goraca A. The protective effect of lipoic acid on selected cardiovascular diseases caused by age-related oxidative stress. Oxid Med and Cell Long. 2015;1-11. Article ID: 313021.

DOI: 10.1155/2015/313021

El-Barky AR, Hussein SA, Mohamed TM. The potent antioxidant alpha lipoic acid. J Plant Chem Ecophysiol. 2017;2(1):1016.

Gu M, Zheng A, Jin J, Cui Y, Zhang N, Che Z-P, Wang Y, Zhan J, Tu W-J. Cardioprotective effects of Genistin in rat myocardial ischemia-reperfusion injury studies by regulation of P2X7/NF-κB pathway. Evid Based Complement Alternat Med. 2016;1-9. Article ID: 5381290.

Wei H, Li H, Wan S-P, Zeng Q-T, Cheng L-X, Jiang L-L, Peng Y-D. Cardioprotective effects of Malavidin agaist isoproterenol-induced myocardial infarction in rats: A mechanistic study. Med Sci Monitor. 2017;23:2007-2016.