Main Article Content
Aim: To investigate the effect of Rhus toxicodendron (30CH) along with different compositions of phytohormones (Auxin and Cytokinin) on the basis of growth and multiplication of explants under optimum temperature under in-vitro conditions.
Study Design: To establish and design the standard protocol for the in-vitro propagation through leaf explant of Scoparia dulcis under stress of phytohormones and homeopathic medicine Rhus toxicodendron (30CH).
Place and Duration of Study: The plant materials were procured from the Herbal Botanical Garden Patna Science College, Department of Botany, Patna University, Patna, Bihar. The experimental part was carried out in Plant Tissue Culture Laboratory, between December 2017 to August 2018 in Department of Botany P.U. Patna.
Methodlogy: The sterilized leaf explants were inoculated into MS media fortified with different phytohormones (Auxin and Cytokinin) and Rhus tox(30CH) under aseptic environmental conditions for the growth and development of callus, embryoids etc.
Result: The explants in MS medium supplemented with auxins phytohormones and Rhus tox(30CH) exhibited that IAA (0.10 to 2.0 mg/l) and BAP (0.10 to 2.5 mg/l) induces green and compact calli. Whereas at 0.30mg/l of IAA and 0.50 mg/l BAP induced brown friable calli. 2,4-D (1.5 mg/l) and Kinetin (1.5-6.5mg/l) concentrations induced brown and friable calli. Rhus tox(30CH) (100 µl/100 ml) enhances proliferation with 2,4-D and Kinetin (1.5/1.5 mg/l.).
Conclusion: After 42 days of culture initiation and establishment the callus was 520.0±1.12 mg in the mixture of 2,4-D and Kinetin (1.5 mg/l) in Rhus tox free medium. Whereas weight of callus were found to be 1092±0.74 mg after 42 days in the same medium of 2, 4-D and Kinetin (1.5/5.5 mg/l) supplemented with Rhus tox (100 µl/100 ml). Hence, the investigation proponded that the Rhus tox (CH30) has increased the rate of callus development and plantlet regeneration.
(Accessed 10 October 2019)
Ratnasooriya WD, Jayakody JRAC, Premakumara GAS and Ediriweera ERHSS. Antioxidant activity of water extract of Scoparia dulcis. Fitoterapia. 2005;76:220-222.
Hayashi T, Okamura K, Kawasaki M, Morita N. Two chemotypes of Scoparia dulcis in Paraguay. Phytochemistry. 1991; 30(11):3671-3620.
Hayashi T.Biologically active diterpenoids from Scoparia dulcis L. (Scrophulariaceae). Studies in Natural Products Chemistry. 2000;21:684- 727.
Osei-Safo D, Chama MA, Mensah A and Waibel R. Hispidulin and other constituents of Scoparia dulcis Linn. Journal of Science and Technology. 2009;29(2):7-14.
Nishino H,Hayashi T, Arisawa M, Satomi Y and Iwashima A. Antitumor-promoting activity of scopadulcic acid b, isolated from the medicinal plant Scoparia dulcis L. Oncology. 1993;50:100-103.
Hayashi K, Hayashi T and Morita N. Cytotoxic and antitumor activity of scopadulcic acid from Scoparia dulcis L. Phytotherapy Research. Clin.1992;6:6- 9.
Madakkannu B and Ravichandran. In vivo immunoprotective role of Indigofera tinctoria and Scoparia dulcis aqueous extracts against chronic noise stress induced immune abnormalities in Wistar albino rats.Toxicology Reports. 2017;4: 484-493.
Pari L, Latha M and Rao CA . Effect of Scoparia dulcis extract on insulin receptors in streptozotocin induced diabetic rats: Studies on insulin binding to erythrocytes. J. Basic. Physiol. Pharmacol. 2004;15:223-240.
Latha M. and Pari.Modulatory effect of Scoparia dulcis in oxidative stress-induced lipid peroxidation in streptozotocin diabetic rats. J. Med. Food. 2004;6(4):1–7.
Latha, M. and Pari L. Effect of an aqueous extract of Scoparia dulcis on plasma and tissue glycoproteins in streptozotocin induced diabetic rats. Pharmazie.2005;60: 151-154.
Hayashi T, Kawasaki M, Miwa Y, Taga T, Morita N. Antiviral agents of plant origin. III. Scopadulin: A novel tetracyclic diterpene from Scoparia dulcis L. Chem. Pharm. Bull.1990;38:945-947.
Ahmed M, Shikha HA, Sadhu SK, Rahman MT and Datta BK. Analgesic, diuretic, and anti-inflammatory principle from Scoparia dulcis. Pharmazie. 2001;56:657-660.
Prabavathy D, Niveditha R. Antibacterial activity of Scoparia dulcis extract on uropathogenic Escherichia coli and Staphylococcus aureus. Research Journal of Chemical Sciences. 2015;6(40):621-626.
Li Y, Ohizumi Y. Search for constituents with neurotrophic factor-potentiating activity from the medicinal plants of paraguay and Thailand. J. Pharm. Soc. Jap. 2004;124:417-424.
Li Y, Chen X, Satake M, Oshima Y and Ohizumi Y. Acetylated flavonoid glycosides potentiating NGF action from Scoparia dulcis. J. Nat. Prod. 2004;67:725-727.
De Farias Freire, SM, Torres LMB, Souccar C, Lapa AJ. Sympathomimetic effects of Scoparia dulcis L. and catecholamines isolated from plant extracts. J. Pharm. Pharmacol.1996;48: 624-628.
Azad MAK, Yokota S, Ohkubo T, Andoh Y, Yahara S and Yoshizawa N. In vitro regeneration of the medicinally woody plant Phellodendron amurense Rupr. Through excised leaves. Plant Cell Tissue. Org. Cult. 2005;80:40-50.
Hassan AKMS and Roy BR. Micropropagation of Gloriosa superba L through high frequency shoot proliferation. Plant Tissue Cult.and Biotech. 2005;15(1): 67-74.
Das R, Hasan MF, Hossain MS and Rahman M. Micropropagation of Centella asiatica L. an important medicinal herb. Progress .Agric. 2008;19(2):51-56.
Janarthanam B, Gopalkrishnam M and Sekar T. Secondary metabolite production in callus cultures of Stevia rebaudiana Bertoni. Bangladesh Journal of Scientific and Industrial Research. 2010;45(3):243-248.
Modolon TA, Boff P, Boff MIC and Miquelluti DJ. Mycelium growth of early tomato blight pathogen, Alternaria solani, subjected to high dilution preparations. Biological Agriculture and Horticulture: An International Journal for Sustainable Production Systems. 2014;31(1):28- 34.
Kumar R and Kumar S. Effect of homeopathic medicines on fungal growth and conodial germination. Indian Phytopathology.1980;33(4):620-621.
Betti L, Trebbi G, Majewsky V, Scherr C, Shah-Rossi D, Jager T and Baumgartner S.Use of homeopathic preparations in phytopathological models and in field trails:a critical review. Homeopathy. 2009;98:244-266.
Bonato CM, Proenca GTD and Reis B. Homeopathic drugs Arsenicum album and sulphur affect the growth and essential oil content in mint (Mentha arvensis L.). Acta Scientiarum. Agronomy. 2009;31(1):101-105.
Gupta VK, Ray JR, Singh VK, Pathak SD, Nayak C and Darokar MP. Dose dependent effect of homeopathic drug zinc sulphate on plant growth using Bacopa monnieri as model system. Indian Journal of Research in Homeopathy. 2014;8(1):19-23.
Kothari SL and Chandra N. Plant regeneration from cultured disc florets of Tagetes erecta. J. Plant. Physiol. 1984; 117:105-108.
Mathur J, Ahuja PS, Mathur A, Kukreja AK and Shah NC. In Vitro propagation of Valeriana wallichii. Planta Med. 1998;54: 82-83.
Pinto JEBP and Lameira Propagation of tissue Cordia verbenacea from tissue culture and micropropagating rooting. In Vitro.1997;33:Pt 2,74A.
Sakthi R and Mohan N. Micropropagation and plant regeneration from leaf and node explants, of Scoparia dulcis. J. Acad. Indus. Res. 2012;1(3):144-147.
Kothari SL and Chandra N. Histology of rhizogenesis and shoot bud formation in cultures of Tagetes erecta L. CurrSci.1986a;55:354-356.
Kothari SL and Chandra N. Plant regeneration in callus and suspension cultures of Tagetes erecta L. (African marigold). J. Plant.Physiol.1986b;122:235-241.
Benavides MP, Caso OH. Plant regeneration and thiophene formation in tissue cultures of Tagetes mendocina. Plant Cell Tiss Org Cul.1993;35:211- 215.
Kothari SL and Chandra N. Morphogenesis in cultures of Tagetes patula. Basic Life Sci.1983;22:423-426.