Main Article Content
Objective: To determine the antioxidant and anti-inflammatory activities of the solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L.
Methods: The flower extract assessed for antioxidant activity using the 1,1–diphenyl-2-picryl-hydrazile (DPPH) radical scavenging assay and the reduced power assay was performed using the Ferric Reducing Capacity (FRC) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) to test the production method of nitric oxide (NO).
Results: The solid powder extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L showed good antioxidant activity in the scavenging DPPH radicals and the FRC assay compared to the standard sample. This powder sample also showed good anti-inflammatory activity in cell viability (LPS induced PBMC) assay and nitric oxide (NO) assay.
Conclusion: These results suggest that the powder sample extracted from the ethyl acetate fraction of the flower Hibiscus vitifolius L has substantial antioxidant and anti-inflammatory activity.
Sarabjot Kaur, Poonam Mondal. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. J Microbiol Exp. 2014;1:1-6.
Nagaveni V Rao. Evaluation of antioxidant potential and qualitative analysis of major polyphenols by RP-HPLC in Nymphaea nouchali Burm. Int J Pharm Pharm Sci. 2010;2:98-104.
Nithya Narayana swamy, Bala krishnan KP. Evaluation of some medicinal plants for their antioxidant properties. Int J Pharm Tech Res. 2011;3:381-5.
Gülçin I. Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino acids. 2007;32(3):431- 438.
Ramassamy C. Emerging role of polyphenolic compounds in the treatment of neurodegenerative diseases: A review of their intracellular targets. Eur J Pharmacol. 2006;545(1):51-64.
Halliwell B. How to characterize an antioxidant: an update. Biochem Soc Symp. 1995;61:73-101.
Brodsky M, Halpert G, Albeck M, Sredni B. The anti-inflammatory effects of the tellurium redox modulating compound, AS 101, are associated with regulation of NF_B signaling pathway and nitric oxide induction in macrophages. Journal of Inflammation. 2010;7:1-8.
Jothibai R, Margret S, Kumaresan, Ravikumar S. A preliminary study on the anti-inflammatory activity of methanol extract of Ulvalactucain rat. J Environ Biol. 2009;5:899-902.
Chandrika M, Chellaram C. Antibacterial properties of leaf extracts of Borreria Hispida from Tamilnadu regions. Der Pharm Chem, 2015;7:389-94.
Chandrika M, Chellaram M. Efficacy of antioxidation and anti-inflammation of the leaf extracts of borreria hispida. Int J Pharm Pharm Sci. 2016;8(8):369- 372.
Anbu Jeba Sunilson John Samuel, Syam Mohan, Dinesh Kumar Chellappan, Ananda rajagopal Kalusa lingam, Saraswathi Ariamuthu. Hibiscus vitifolius (Linn.) root extracts shows potent protective action against anti-tubercular drug induced hepatotoxicity. Journal of Ethnopharmacology. 2012;141:396–402.
Rao KS, Lakshminarayana G. Fatty acid compositions of seed oils of seven Hibiscus species of Malvaceae. Journal of the American Oil Chemists’ Society. 1985; 62:714–715.
Parmar NS, Ghosh MN. Effect of gossypin a flavonoid, on the formation of galactose-induced cataracts in rats. Experimental Eye Research. 1979;29:229–232.
Arnold HJ, Gulumian M. Pharmacopoeia of traditional medicine in Venda. Journal of Ethnopharmacology. 1984;12:35–74.
Gautam P, Flora SJS. Oral supplementation of gossypin during lead exposure protects alteration in heme synthesis pathway and brain oxidative stress in rats. Nutrition. 2010;26:563-570.
Harborne JB. Phytochemical Methods, A guide to modern techniques of plant analysis (3rd Ed.), Springer Pvt. Ltd., New Delhi, India; 1998.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199–1200.
Adedapo AA, Jimoh FO, Koduru S, Afolayan AJ, Masika PJ. Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. B.M.C. Complement. Altern. Med. 2008;8(53):1-8.
Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci. 2010;2(1):146-56.
Benzie IEF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem. 1996;239:70-76.
Satheesh kumar DR, Pooja M, Harika K, Haswitha E, Nagabhushanamma G and Vidyavathi N. In-vitro antioxidant activities, total phenolics and flavonoid contents of whole plant of Hemidesmus indicus Linn. Asian J Pharm Clin Res. 2013;6(2):249-251.
Christopher Seng Hong Lim, Siew Lin Lim. Ferric reducing capacity versus ferric reducing antioxidant power for measuring total antioxidant capacity. Laboratory Medicine. 2013;44(1):51-55.
Jenny M, Klieber M, Zaknun D, Schroecksnadel S, Kurz K , Ledochowski MSchennach H, Dietmar Fuchs. In vitro testing for anti-inflammatory properties of compounds employing peripheral blood mononuclear cells freshly isolated from healthy donors. Inflamm. Res. 2011;60: 127–135.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Analytical Biochemistry. 1982;126:131-138.
Saravanan S, HairulIslam VI, Thirugnanasambantham K, Pazhanivel N , Raghuraman N , Gabriel Paulraj. Ignacimuthu MS. Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1β induced rat fibroblast-like synoviocytes. Inflamm. Res. 2014;63:451–462.
Ignacio SRN, Ferreira JLP, Almeida MB, Kubelka CF. Nitric oxide production by murine peritoneal macrophages in vitro and in vivo treated with Phyllanthustenellus extracts. J Ethnopharmacol. 2001;74:181–7.
Richa Upadhyay, Jitendra kumar Chaurasia, Kavindra Nath Tiwari, Karuna Singh. Antioxidant property of aerial parts and root of phyllanthusfraternus webster, an important medicinal plant. Scientific World J. 2014;2014:2-5.
Sumanya H, Lavanya R, Umamaheswara reddy R. Evaluation of in vitro anti-oxidant and anti-arthritic activity of methanolic extract of marine green algae caulerpa racemosa. Int J Pharm PharmSci. 2015; 7(7):340-343.
Saha MR, Hasan SMR, Akter R, Hossain MM, Alam MS, Alam MA, Mazumder MEH. In vitro free radical scavenging activity of methanol extract of the Leaves of mimusopselengilinn. Bangl J Vet Med. 2008;6(2):197–202.
Forstermann U, Kleinert H. Nitric oxide synthase: Expression and expressional control of the three isoforms, NaunynSchmiedebergs Arch. Pharmacol. 1995;352:351–364.