European Journal of Medicinal Plants

  • About
    • About the Journal
    • Submissions & Author Guideline
    • Accepted Papers
    • Editorial Policy
    • Editorial Board Members
    • Reviewers
    • Propose a Special Issue
    • Reprints
    • Subscription
    • Membership
    • Publication Ethics and Malpractice Statement
    • Digital Archiving Policy
    • Contact
  • Archives
  • Indexing
  • Publication Charge
  • Submission
  • Testimonials
  • Announcements
Advanced Search
  1. Home
  2. Archives
  3. 2019 - Volume 29 [Issue 1]
  4. Original Research Article

Submit Manuscript


Subscription



  • Home Page
  • Author Guidelines
  • Editorial Board Member
  • Editorial Policy
  • Propose a Special Issue
  • Membership

Anti-Acetylcholinesterase Compounds Isolated from the Leaves of Kigelia africana (LAM) Benth (Bignoniaceae)

  • John A. Falode
  • Olamide O. Crown
  • Samson O. Famuyiwa
  • Christianah A. Elusiyan
  • Ifedayo V. Ogungbe
  • Afolabi C. Akinmoladun
  • Mary T. Olaleye
  • Afolabi A. Akindahunsi

European Journal of Medicinal Plants, Page 1-9
DOI: 10.9734/ejmp/2019/v29i130148
Published: 20 September 2019

  • View Article
  • Download
  • Cite
  • Statistics
  • Share

Abstract


Acetylcholinesterase (AChE) is an enzyme that is involved in the breakdown of some neurotransmitters. Its inhibition is one of the treatment strategies employed in the management Alzheimer diseases. Flavonoids isolated from the leaves of Kigelia africana were investigated for their comparative AChE inhibition.


The extract of the leaves was subjected to vacuum liquid chromatography (VLC) to obtain four fractions using n-hexane (n-hex, 100%), n-hexane/dichloromethane (hex/DCM, 1:1), dichloromethane/ethyl acetate (DCM/EtOAc, 1:1) and ethyl acetate/methanol (EtOAc/MeOH, 1:1). The four fractions were subjected to AChE inhibitory study with DCM/EtOAc (1:1) fraction showing the highest inhibitory activity. Three flavonoids were isolated from this fraction and their structures were elucidated and characterised using 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) techniques. Their spectroscopic data compared well with literature.


The compounds demonstrated considerable inhibition of AChE activity with luteolin (1), rutin (2) and quercetin (3) that showed IC50 of 945.0, 282.1, 254.8 μg/ml respectively as against the IC50 of 38.93 μg/ml for rivastigmine, a well-known cholinesterase inhibitor. Compound 3 showed 17.89 ± 0.57 and 7.70 ± 0.64 μ/l/mg protein at 200 and 400 μg/ml respectively, for AChE activity as against 10.37 ± 0.99 and 6.24 ± 1.24 μ/l/mg protein showed by rivastigmine at 200 and 400 μg/ml respectively.


This study showed that the constituents responsible for the AChE inhibition in the crude extract as reported by Falode et al., 2017 resided in the DCM/EtOAc (1:1) fraction. The structure-activity relationship of the flavonoids revolves around substitution in position 3 of the compounds. 


Keywords:
  • Kigelia Africana
  • flavonoids
  • structural elucidation
  • dementia
  • acetylcholinesterase inhibition.
  • Full Article PDF
  • Review History

How to Cite

Falode, J. A., Crown, O. O., Famuyiwa, S. O., Elusiyan, C. A., Ogungbe, I. V., Akinmoladun, A. C., Olaleye, M. T., & Akindahunsi, A. A. (2019). Anti-Acetylcholinesterase Compounds Isolated from the Leaves of Kigelia africana (LAM) Benth (Bignoniaceae). European Journal of Medicinal Plants, 29(1), 1-9. https://doi.org/10.9734/ejmp/2019/v29i130148
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
  • Abstract View: 2305 times
    PDF Download: 920 times

Download Statistics

  • Linkedin
  • Twitter
  • Facebook
  • WhatsApp
  • Telegram
Make a Submission / Login
Information
  • For Readers
  • For Authors
  • For Librarians
Current Issue
  • Atom logo
  • RSS2 logo
  • RSS1 logo


© Copyright 2010-Till Date, European Journal of Medicinal Plants. All rights reserved.