In vitro Investigation of Phytoconstituents, GC-MS, TLC, Antioxidant activity, Total Phenolic & Flavonoid Contents from Aegle marmelos L (Bael) Leaves Extract
Ramesh Kumar *
Department of Biochemistry, Bundelkhand University, Jhansi (UP)- 284128, India.
Smrati Sharma
Department of Biochemistry, Bundelkhand University, Jhansi (UP)- 284128, India.
Sidhanshu Kumar
Department of Biochemistry, Bundelkhand University, Jhansi (UP)- 284128, India.
Dinesh Kumar
Department of Chemistry, Bipin Bihari College, Jhansi (UP), India.
Rekha Lagarkha
Department of Chemistry, Bundelkhand University, Jhansi (UP)- 284128, India.
Sanjay Kumar
Department of Microbiology, Bundelkhand University, Jhansi (UP)- 284128, India.
Maneesha Pandey
Biochemistry Discipline, School of Sciences, Indira Gandhi National Open University, New Delhi 110068, India.
*Author to whom correspondence should be addressed.
Abstract
The Rutaceae family includes Aegle marmelos L., a moderately sized aromatic tree that is also known as Bael, Bengal quince, golden apple, Japanese bitter orange, stone apple, or wood apple. It is mostly found in tropical or subtropical regions. Fever, seminal weakness, nausea, vomiting, swellings, diarrhea, dyspepsia, and intermittent fever are the most common ailments that bael leaves are used to treat. Because of their high phenolic and flavonoid content, leaves have anti-allergic, anti-inflammatory, anti-microbial, anti-thrombotic, cardioprotective, and vasodilatory properties. Therefore, the aim of the present study was to determine the phytochemical components, antioxidant capacity, GC-MS analysis, total phenolic & flavonoid contents in the methanolic extracts of A. marmelos leaves. Various secondary metabolites were observed in the aqueous and methanolic A. marmelos leaf extracts. TLC analysis of methanolic extracts revealed five distinct spots having different Rf values. Total phenolic content was found to be 33 ± 7.62 mg GAE/g, flavonoid content was 307.8 ± 130.12 mg QE/g. The highest antioxidant activity of methanolic extract of A. marmelos leaves was obtained at 1000 µg/ml conc. and it was dose dependent. Many biologically active compounds were present as analysed by GC-MS. The highest peak area of Perylo[1,12-def]-1,3-dioxepin-5,11-dione, 6,12-dihydroxy-8,9-bis(2-hydroxypropyl)-7,10-dimethoxy-, stereoisomer (3.502%) was observed.
Keywords: A. marmelos, ascorbic acid, antioxidant activity, GC-MS, total phenolic content, total flavonoid content