Antihyperlipidemic Bioactivity Guided Isolation and Structural Elucidation of Isolated Phytoconstituents from Convolvulus pluricaulis Choisy

Deepa Iyer *

Madhya Pradesh Council of Science and Technology, Vigyan Bhawan, Nehru Nagar, Bhopal- 462003, M.P., India.

Nimisha Jain

Institute of Pharmacy, Rabindranath Tagore University, Bhopal, 464993, M.P., India.

U. K. Patil

Phytomedicine and Natural Product Research Laboratory, Department of Pharmaceutical Sciences, Dr. H.S. Gour Vishwavidyalaya (A Central University), Sagar- 470003, M.P., India.

*Author to whom correspondence should be addressed.


Background: Atherosclerosis is a crucial element in the origination of coronary heart disease (CHD). Lipid aggregation inside the coronary arteries walls lead to the occurrence of hyperlipidemia. Aim: The research was conducted for assessing the lipid lowering effect of ethanol extract and chloroform fraction from Convolvulus pluricaulis Choisy. aerial parts in Triton induced hyperlipidemic rat model. 

Methodology: The active fraction was obtained from antihyperlipidemic bioactivity. The phytocomponents were separated from the active fraction using flash chromatography technique resulting in the isolation of n-hexatriacontane; 9-octadecenoic acid- octyl ester; 12, 14-heptacosanedione; dodecyl-octadeca-9,12-dienoate; tetracosanyl 9-hexadecenoate; heptacosan-14-ol; stigmasta- 5, 22- dien-3 beta-ol and stigmast-5-en-3 beta-ol. The phytocomponent structures were established by spectral data analysis. Intraperitoneal (i.p.) injection of Triton WR- 1339 at a dose of 400 mg/kg body weight (b.w.) was given to the animals. The ethanol extract at a dose of 200 mg/kg b.w. and chloroform fraction at a dose of 400 mg/kg b.w. were administered orally in rats after 24 hours of the Triton administration.

Results: The study resulted in dose dependent inhibition of triglycerides (p <0.01), total cholesterol (p <0.05), Low density lipoprotein level (p <0.05) while significant increased HDL (p <0.01) level. Alteration in lipid profile in Triton treated rats was restored and found more effective in the chloroform fraction probably due to the presence of phytocomponents. The study elucidated different phytocomponents isolated from the active chloroform fraction of C. pluricaulis.

Conclusion: Treatment with extract and fraction manifested significant decrease in triglyceride level. HDL exerts an inhibitory action in the etiology of atherosclerosis. It is considered as a beneficial lipoprotein and its lower level leads to the greater risk of CHD. It may be concluded that the isolated components were responsible for the lipid lowering level of active fraction.




Keywords: Convolvulus pluricaulis, anti-hyperlipidemic bioactivity, flash chromatography, isolated phytoconstituents

How to Cite

Iyer , Deepa, Nimisha Jain, and U. K. Patil. 2023. “Antihyperlipidemic Bioactivity Guided Isolation and Structural Elucidation of Isolated Phytoconstituents from Convolvulus Pluricaulis Choisy”. European Journal of Medicinal Plants 34 (12):11-21.


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Kirtikar KR, Basu BD. Indian Medicinal Plants. Sri Satguru Publications, India, 1999;1738-1739.

Chatterjee A. Treatise of Indian Medicinal Plants. Council for Scientific and Industrial Research, India. 1990;327.

Nahata A, Patil UK, Dixit VK. Anxiolytic activity of Evolvulus alsinoides and Convolvulus pluricaulis in rodents. Pharm Biol. 2009;47:444-451.

Asolkar LV, Kakkar KK, Chakre OJ. Second Supplement to Glossary of Indian Medicinal Plants with Active Constituents. NISCAIR, India. 1992;965.

Lilly G, Dev K, Sudipta C, Shrivastava KK, Sawhney RC, Selvamurthy W. Immuno- modulatory effects of agents of plant origin. Biomed Pharmaco. 2003;7:296-300.

Siripurapu KB, Gupta P, Bhatia G, Maurya R, Nath C, Palit G. Adaptogenic and antiamnesic properties of Evolvulus alsinoides in rodents. Pharmacol Biochem Behav. 2005;81:24- 432.

Mukherjee B, Seal T, Tripathi PC, Dajas F. Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. J Ethnopharmacol. 2003;84:131-138.

Prajapati ND, Purohit SS, Sharma AK, Kumar T. A Handbook of Medicinal Plants. Agrobios, India. 2003;234- 235.

Friedman M, Bayer SO. The mechanism underlying hypocholesterolemia induced by Triton WR 1339. Am J Physiol. 1957;190:439- 445.

Nayak B, Roy S, Roy M, Mitra A, Karak K. Phytochemical, antioxidant and antimicrobial screening of Suaeda maritime against human pathogens and multiple drug resistant bacteria. Ind J Pharm Sci. 2018;80:26–35.

Kumari C, Deepalakshmi J. Qualitative and GC-MS analysis of phytoconstituents of Parthenium hysterophorus. Res J Pharmacog Phytochem. 2017;9:105- 110.

Patil UK, Dixit VK. Hypolipidemic activity of Cassia tora L. seeds. J Ethnopharmacol. 2004;90:249-252.

Ghule BV, Ghante MH, Saoji AN, Yeole PG. Hypolipidemic and antihyperlipidemic effects of Lagenaria siceraria Mol. fruit extracts. Ind J Exp Biol. 2006;44:905- 909.

Takahashi Y, Zhu H, Yoshimoto T. Essential roles of lipoxygenases in LDL oxidation and development of atherosclerosis. Antiox Redox Signal. 2005;7:425–431.

Parab RS, Mengi SA. Hypolipidemic activity of Acorus calamus L. in rats. Fitoterapia. 2002;73:451–455.

Ahmed I, Lakhani MS, Gillet M et al. Hypotriglyceridemic and hypocholesterolemic effects of anti-diabetic Momordica charantia fruit extract in streptozotocin-induced diabetic rats. Diabetes Res Clin Pract. 2001;51:155–161.

Aikawa M, Libby P. The vulnerable atherosclerotic plaque:Pathogenesis and therapeutic approach. Cardiovas Pathol. 2004;13:125–138.

Sairam K, Rao CV, Goel RK. Effect of Convolvulus pluricaulis on gastric ulceration and secretion in rats. Ind J Exp Biol. 2001;39:350-354.

Dhingra D, Valecha R. Evaluation of the antidepressant-like activity of Convolvulus pluricaulis in the mouse forced swim and tail suspension tests. Inter Sci Lit. 2007;13:155-161.

Bihaqi SW, Sharma M, Singh AP, Tiwari M. Neuroprotective role of Convolvulus pluricaulis on aluminium induced neurotoxicity in rat brain. J Ethnopharmacol. 2009;124:409–415.

Neeraj KS, Mishra SH. Review on ethnomedicinal uses and phytopharmacology of memory boosting herb Convolvulus pluricaulis. Aus J Med Herb. 2010;22:19-25.