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Background and Objectives: Medicinal plants have become popular in the search ofa cure for various hepatopathies with a considerable number of herbal products being used for the management of liver ailments. However, most, including Hura crepitans (HC), have not been toxicologically evaluated. The present study was aimed at determining the phytoconstituents of HC, their antioxidant potentials and safety in animals.
Place and Duration: The study was performed at the Department of Biochemistry and Biotechnology, KNUST, Kumasi and subacute toxicity studies lasted for 14 days.
Materials and Methods: Hydroethanolic extracts and fractions of the leaves and stem back of HC were assessed for their phytochemical constituent,DPPH and FRAP scavenging activities and total phenolic and total flavonoid content. The acute and sub acute oral toxicity of the hydroethanolic stem back of HCin animals was evaluated using standard methods to establish the LD50, effect on weight, haematological and biochemical parameters.Phytochemical characterization was achieved using standard methods.
Results: The study showed that HC contained tannins, saponins, flavonoids, coumarins, glycosides, and triterpenoids. The hydroethanolic extract of the stem bark (EES) showed the greatest antioxidant potential. The LD50 estimate of the hydroethanolic extract of stem back was greater than 5000 mg/kg body weight/oral route, while the doses used in this study up to 1000 mg/kg body weight/oral route was safe. The extract generally did not cause any significant decrease in the weight of animals nor were there significant differences in relative organ weights in treated animals compared to the normal group. Haematology, liver function, kidney function, and serum electrolyte parameters were within the normal ranges in treated animals when compared to the control group.
Conclusion: The results showed that oral administration of hydroethanolic extract of Hura crepitansin animals up to 1000 mg/kg body weight is considered safe as it causes no significant derangement in clinical and haemato-biochemical parameters measured.
Roberts H. ACCRA: A Way forward for mental health care in Ghana?” Lancet. 2001;357(9271):1859.
WHO.General guidelines for methodologies on research and evaluation of traditional medicine. Geneva: World Health Organisation; 2000.
Okigbo RN, Mmeka EC. An appraisal of phytomedicine in Africa. KMITLScience and Technology Journal. 2006;6(2):83- 94.
Cameron A, Ewen M,Ross-Degnan D,Ball D,LaingR.Medicine prices, availability, and affordability in 36 developing and middle-income countries: A Secondary Analysis. Geneva: The World Health Organisation; 2008.
Akindele AJ, Ezenwanebe KO, Anunobi CC, Adeyemi OO. Hepatoprotective and in vivo antioxidant effects of Byrsocarpus coccineus Schum and Thonn. (Connaraceae). J. Ethnopharmacol. 2010; 129(1):46-52.
Amat N, Upur H, Blažeković B. In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. J. Ethnopharmacol. 2010;131(2):478-484.
Bishayee A, Sarkar A, Chatterjee M.Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver. J. Ethnopharmacol.1995;47(2):69-74.
Suja S, Latha P, Pushpangadan P, Rajasekharan S. Evaluation of hepatoprotective effects of Helminthostachys zeylanica (L.) Hook against carbon tetrachloride-induced liver damage in Wistar rats. J. Ethnopharmacol. 2004;92(1):61-66.
Opoku A, Ndlovu I, Terblanche S, Hutchings A. In vivo hepatoprotective effects of Rhoicissus tridentata subsp. cuneifolia, a traditional Zulu medicinal plant, against CCl4-induced acute liver injury in rats. S. Afr. J. Bot. 2007;73(3): 372-377.
Dahiru D, William E, Nadro M. Protective effect of Ziziphus mauritiana leaf extract on carbon tetrachloride-induced liver injury.Afr. J. Biotechnol. 2005;4(10):1177-1179.
Adewusi E, Afolayan A. A review of natural products with hepatoprotective activity.J Med. Plant Res. 2010;4(13):1318-1334.
Singh A, Handa SS. Hepatoprotective activity of Apium graveolens and Hygrophila auriculata against paracetamol and thioacetamide intoxication in rats. J Ethnopharmacol. 1995;49(3):119-126.
Jafri M, Subhani MJ, Javed K, Singh S.Hepatoprotective activity of leaves of Cassia occidentalis against paracetamol and ethyl alcohol intoxication in rats. J Ethnopharmacol. 1999;66(3):355-361.
Olaleye M, Adegboye O, Akindahunsi A. Alchornea cordifolia extract protects wistar albino rats against acetaminophen-induced liver damage. Afr. J. Biotechnol. 2006;5(24):2439-2445.
Owojuyigbe OS, Firempong CK, Larbie C, Komlaga G, Emikpe BO. Hepatoprotective Potential of Hura crepitans L.: A Review of Ethnomedical, Phytochemical and Pharmacological Studies. Journal of Complementary and Alternative Medical Research. 2020;9(2):1-10.
Jernigan KA. Barking up the same tree: A comparison of ethnomedicine and canine ethnoveterinary medicine among the Aguaruna. J. Ethnobiol. Ethnomed. 2009; 5:33.
Burkill HM. The useful plants of west tropical Africa, 1-3: Royal Botanic Gardens, Kew; 1995.
Oderinde RA, Ajayi I, Adewuyi A. Preliminary Toxicological Evaluation and Effect of the Seed oil of Hura crepitans and Blighia unijugata Bak on the lipid profile of rat. EJEAFChe. 2009;8(3):209-217.
Gajdács M, Albericio F. Antibiotic resistance: From the bench to patients. Antibiotics. 2019;8(3):129.
Efferth T, Kaina B. Toxicities by herbal medicines with emphasis to traditional Chinese medicine. Curr Drug Metab. 2011; 12(10):989-996.
Gajdács M. The concept of an ideal antibiotic: implications for drug design molecules. 2019;24(5):892.
Gregory Cope W. Exposure classes, toxicants in air, water, soil, domestic and occupational settings. In: Hodgson E, editor. A textbook of modern toxicology. 3rd Ed. New Jersey: John Wiley and Sons, Inc; 2004.
Organisation for economic co-operation and development. OECD guideline for testing of chemicals: acute oral toxicity – acute toxic class method. in environmental health and safety monograph series on testing and assessment. 243:1-2, OECD Publishing, Paris, France; 2001.
Walum E. Acute Oral Toxicity. Environ. Health Perspect.1998;106(2):497–502.
Twaij HA, Kery A, Al-Khazraji NK. Some pharmacological, toxicological and phytochemical investigations on Centaurea phyllocephala. JE thnopharmacol. 1983;9(2-3):299-314.
Kedare SB, Singh RP, Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011;48(4):412–422.
Benzie IFF, Davaki M. The ferric reducing/antioxidant power (FRAP) assay for non‐enzymatic antioxidant capacity: Concepts, procedures, limitations and applications. In Apak R, Capanoglu E, Shahidi F. (eds) Measurement of Antioxidant Activity & Capacity: Recent Trends and Applications; 2018.
Yen GC, Lai HH, Chou HY. Nitric oxide scavenging and antioxidant effects of Urariacrinita root. Food Chem. 2001;74: 471-478. DOI:https://doi.org/10.1016/S0308-8146(01)00165-0
Chang CC, Yang MH, Wen HM, Chern. JC. Estimation of total flavonoid content in Propolis by two complementary colorimetric methods. J Food Drug Ana. 2002;10:178-182.
Arthur FKN, Woode E, Terlabi EO, Larbie C. Evaluation of acute and subchronic toxicity of Annona muricata Linn. aqueous extract in animals. European Journal of Experimental Biology. 2011;1(4):115-124.
National Research Council. Guide for care and use of laboratory animal. national academic press, Washington, USA, 8th Edition; 2001.
Kar A. Pharmaocgnosy and Pharmacobiotechnology (Revised-expanded second edition). New age international limited publishers New Delhi. 2007;332-600.
Leal LKAM, Ferreira AAG, Bezzera GA, Matos FJA, Viana GSB. Anticonceptive, anti-inflammatory and bronchodilator activities of Brazilian medicinal plants containing coumarin: A comparative study. J Ethnopharmacol. 2000;70(20):151-159.
Martinez MJA, Lazaro RM, del Olmo LMB, Benito PB.Anti-infectious activity in the Anthemideae tribe. Studies in Natural Products Chemistry. 2008;35:445-516.
Milugo TK, Omosa LK, Ochanda JO, Owuor BO, Wamunyokoli FA, Oyugi JO, Ochieng JW. Antagonistic effect of alkaloids and saponins on bioactivity in the quinine tree (Rauvolfia caffra sond.): Further evidence to support biotechnology in traditional medicinal plants.BMC Complement Altern Med. 2013;26(13):285.
Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends in Plant Sci. 1997; 2(4):152-159.
Jayasri M, Mathew L, Radha A. A report on the antioxidant activity of leaves and rhizomes of Costuspictus D. Don. Int J Integr Biol. 2009;5(1):20-26.
Liu RH. Potential synergy of phytochemicals in cancer prevention: Mechanism of action. J Nutr. 2004; 134(12):3479S-3485S.
Agbedahunsi JM, Fakoya FA, Adesanya SA. Studies on the anti-inflammatory and toxic effects of the stem bark of Khaya ivorensis (Meliaceae) on rats. Phytomedicine. 2004;11(6):504-508.
Witaicenis A, Roldao EF, Seito LN, Rocha NP, Di Stasi LC. Pharmacological and toxicological studies of Drimys angustifolia Miers (Winteraceae).J Ethnopharmacol. 2007;111(3):541-546.
Raza M, Al-Shabanah O, El-Hadiyah T, Al-Majed A. Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Scientia Pharmaceutica. 2002;70:135-145.
Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. A 90-day oral gavage toxicity study of d-methylphenidate and d, l-methylphenidate in Sprague–Dawley rats. Toxicology. 2002;179(3):183-196.
Michael B, Yano B, Sellers RS, Perry R, Morton D, Roome N, Johnson JK, Schafer K, Pitsch S. Evaluation of organ weights for rodent and non-rodent toxicity studies: A review of regulatory guidelines and a survey of current practices. Toxicol. Pathol. 2007;35(5):742-750.
Ajagbonna O, Onifade K, Suleiman U. Haematological and biochemical changes in rats given water extract of Calotropis procera. Sokoto J. Vet. Sci. 1999;1(1):36-42.
Etim NN, Williams ME, Akpabio U, Offiong EEA. Haematological Parameters and Factors Affecting Their Values. Agricultural Science. 2014;2(1):37-47.
Ashafa A, Yakubu M, Grierson D, AfolayanA.Effects of aqueous extract from the leaves of Chrysocoma ciliata L. on some biochemical parameters of Wistar rats. Afr. J. Biotechnol. 2009;8(8):1425-1430.
Yamamotoya T, Nakatsu Y, Matsunaga Y, Fukushima T, Yamazaki H, Kaneko S, Fujishiro M, Kikuchi T, Kushiyama A, Tokunaga F, Asano T, Sakoda H.Reduced SHARPIN and LUBAC formation may contribute to CCl4-or acetaminophen-induced liver cirrhosis in mice. Int. J. Mol. Sci. 2017;18(2):326.
Brautbar N, Williams J. Industrial solvents and liver toxicity: Risk assessment, risk factors and mechanisms.Int. J. Hyg. Environ. Health. 2002;205(6):479-491.
Whelton A, Watson A, Rock R.Nitrogen metabolites and renal function. Tietz Textbook of Clinical Chemistry, 2nd Ed. Burtis CA, Ashwood ER, eds. Philadelphia: WB Saunders Company. 1994;1513-1575.
Field M, Block JB, Levin R, Rall DP. Significance of blood lactate elevations among patients with acute leukemia and other neoplastic proliferative disorders. Am. J. Med. 1966;40(4):528.