Arbuscular Mycorrhizal and Root Colonizing Dark Septate Endophyic Fungal Associations in Urginea indica and Urginea wightii Accessions

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B. Mohana
Shiva Kameshwari
Hanumanth Rao

Abstract

Urginea indica belongs to Hyacinthaceae family. It is also known as Indian squill, commonly called as wild Onions. The over exploitation and habitat degradation has resulted in the loss of habitat and it has caused genetic depletion and loss of genetic diversity. Immediate Measures have to be undertaken for conservation, to save this economically important medicinal plant. The present investigation is an attempt to highlight the occurance of arbuscular mycorrhizal (AM) and Dark Septate Endophyte (DSE) fungal association found in the root bulbs of Urginea indica. 8 Accessions of Urginea was examined in Urginea indica kunth and Urginea wightii accessions, collected from various regions of Karnataka and South India. Fungal Symbiosis was present in all the accessions, with 100% root infection. Urginea has DSE fungal association and AM Association.

This is the first report on the DSE fungal association in Urginea indica kunth. In addition to the prevalance of AM fungal symbiosis. The role of DSE is still in infancy. Though noclear relationship between AM and DSE fungal colonizations was recognized. Our studies suggests the coexistence of AM and DSE fungal colonizations are common terrestrial habitats especially the xerophytic environment. DSE-plant symbiosis should not be limited to nutritional uptake but mediates other parameters such as drought resistance, stress tolerance and herbivore resistance. Our results show 100% infection by AM and DSE fungal colonies. Infection levels were high throughout the year, the highest levels occurring in the most nutrient-stressed situations. Their occurrence and variaton in different accessions is discussed below.

Keywords:
Arbuscular mycorrhiza, dark septate endophytic fungi, Urginea.

Article Details

How to Cite
Mohana, B., Kameshwari, S., & Rao, H. (2020). Arbuscular Mycorrhizal and Root Colonizing Dark Septate Endophyic Fungal Associations in Urginea indica and Urginea wightii Accessions. European Journal of Medicinal Plants, 31(1), 11-16. https://doi.org/10.9734/ejmp/2020/v31i130200
Section
Original Research Article

References

Shiva Kameshwari MN, Lakshman AB, Paramasivam G. Biosystematics studies on medicinal plant Urginea indica Kunth. liliaceae - A review. Int. J. of Pharm. & Life Sci. 2012;3(1):1394-1406.

Chittoor MS, Roger Binny AJ, Yadlapalli S, Cheruku A, Dandu C, Nimmanapalli Y. Anthelmintic and antimicrobial studies of Drimia indica (Roxb.) Jessop. bulb aqueous extracts. Journal of Pharmacy Research. 2012;5(5):3677-3686.

Joshi KC, Negi MS, Tiple AD. Achanakmar-Amarkantak biosphere reserve. Biosphere Reserve Information Series (BRIS), 2(1-2). Tropical Forest Research Institute Jabalpur. 2010;1-158.

Beena KR, Raviraja NS, Arun AB, Sridhar KR. Diversity of arbuscular mycorrhizal fungi on the coastal sand dunes of west coast of India. Current Science. 2000;79: 1459-1466.

Vesicular-arbuscular (VA) mycorrhizal status of some aquatic and marshy plants. Acta Botanica Indica. 1993;21:161–171.

Muthukumar T, Prabha K. Arbuscular mycorrhizal and septate endophyte fungal associations in lycophytes and ferns of South India. Symbiosis. 2013;59(1):15–33.

Muthukumar T, Udaiyan K. Seasonality of vesicular arbuscular mycorrhizae in sedges in a semi-arid tropical grassland. Acta Oecologica. 2002;23(5):337–347. Muthukumar T, Udaiyan K, Shanmughavel P. Mycorrhiza in sedges—an overview. Mycorrhiza. 2004;14(2):65–77.

Gokhale MV, Shaikh SS, Chavan NS. Floral survey of wet coastal and associated ecosystems of Maharashtra. Indian Journal of Geo Marine Sciences. 2011;40(5):725-730.

Dickson S, Smith FA, Smith SE. Structural differences in arbuscular mycorrhizal symbioses: More than 100 years after Gallaud, where next? Mycorrhiza. 2007;17(5):375–393.

Muthukumar T, Udaiyan K. Arbuscular mycorrhizas of plants growing in the Western Ghats region, Southern India. Mycorrhiza S, Dharmarajan K, Kannan, Lakshminarasimhan C. 2000;15:297–313.

Gerdemann JW, Nicolson TH. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc. 1963;46:235-244.

Shiva Kameshwari MN. Biosystematics studies on Urginea indica Kunth. Liliaceae. (Abs) Nat. Conf. on Forest Biodiversity Resources: Exploitation Conservation & Management, 21-22 March CBFS, Madurai Kamaraj University: Madurai. 2006;24-25.

Gerdemann JW. Vesicular-arbuscular mycorrhizae formed on maize and tuliptree by Endogone fasciculate. Mycologia. 1965;57:562–575.

Jumpponen A, Trappe JM. Dark-septate root endophytes: A review with special reference to facultative biotrophic symbiosis. New Phytol. 1998;140:295–310.

Nicolson TH. The mycotrophic habit in grasses. Ph.D. Thesis. University of Nottinghana; 1955.

Brundrett M. Mycorrhizal associations and other means of nutrition of vascular plants: Understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant and Soil. 2009;320(1-2): 37–77.

Lukešová T, Kohout P, Větrovský T, Vohník M. The potential of dark septate endophytes to form root symbioses with ectomycorrhizal and ericoid mycorrhizal middle European forest plants. PLoS One. 2015;10(4).

Abbott LK, Robson AD. The role of VAM fungi in, agriculture and the selection of fungi for inoculation. Aust. J. Agric. Res. 1982;33:389–408.

Radhika KP, Rodrigues BF. Arbuscular mycorrhizae in association with aquatic and marshy plant species in Goa, India. Aquatic Botany. 2007;86(3):291–294.

Dickson S. The Arum-Paris continuum of mycorrhizal symbioses. New Phytologist. 2004;163(1):187–200.