Endothelium-Independent Vasorelaxant Effects of Anthocyanins-Enriched Extract from Odontonema strictum (Nees) Kuntze (Acanthaceae) Flowers: Ca2+ Channels Involvement

Main Article Content

Mathieu Nitiéma
Moumouni Koala
Lazare Belemnaba
Jean Claude W. Ouédraogo
Salfo Ouédraogo
Félix Bondo Kini
Sylvin Ouédraogo
Innocent Pierre Guissou

Abstract

Aims: We aimed in this study to investigate the mechanisms of the vasorelaxation effect caused by the anthocyanins-enriched extract of Odontonema strictum flowers.

Study Design: Anthocyanins-enriched extract of Odontonema strictum flowers and vasorelaxantes activities of mice aortic rings.

Place and Duration of Study: The flowers of Odontonema strictum (Nees) Kuntze (Acanthaceae) were collected in January 2015 at the “Institut de Recherche en Sciences de la Santé (IRSS)” experimental station in Ouagadougou. The experiments were conducted in October - November 2018 at the department of Medicine and Traditional Pharmacopeia-Pharmacy (MEPHATRA-PH)/IRSS.

Methodology: The extract was enriched in anthocyanins using Amberlite XAD-7 non-ionic resin column. The vasorelaxant activity of anthocyanins-enriched extract of O. strictum flowers (OSF) was tested using isolated organ-chamber technique with mice aorta rings.

Results: OSF showed concentration-dependent relaxant effects on mice endothelium intact or denuded aortic rings pre-contracted with U46619 (10-7 M) and KCl (80 mM). OSF induced relaxation in the mice aortic rings by stimulating smooth muscle cells. The vasorelaxant effect of OSF (10-1000 µg/mL) was similar in endothelium-intact and endothelium-denuded aortic rings. The maximum relaxant effect was 93.78 ± 4.69% and 92.30 ± 3.19% for endothelium-intact and endothelium-denuded aortic rings, respectively. Moreover, after incubation of the aorta rings with OSF (400 µg/mL) or vehicle (0.02% of DMSO) in PSS, OSF blocked the contraction through mechanism involving inhibition of CaCl2 and U46619 effect.

Conclusions: The present study provides a pharmacological evidence for the antihypertensive medicinal use of Odontonema strictum by highlighting its vasorelaxant activity.

Keywords:
Odontonema strictum, flowers, endothelium-independent, vasorelaxant, calcium channels

Article Details

How to Cite
Nitiéma, M., Koala, M., Belemnaba, L., Ouédraogo, J. C., Ouédraogo, S., Kini, F., Ouédraogo, S., & Guissou, I. (2019). Endothelium-Independent Vasorelaxant Effects of Anthocyanins-Enriched Extract from Odontonema strictum (Nees) Kuntze (Acanthaceae) Flowers: Ca2+ Channels Involvement. European Journal of Medicinal Plants, 29(3), 1-11. https://doi.org/10.9734/ejmp/2019/v29i330155
Section
Original Research Article

References

Vergara-Galicia J, Ortiz-Andrade R, Rivera-Leyva J, Castillo-España P, Villalobos-Molina R, Ibarra-Barajas M, Gallardo-Ortiz I, Estrada-Soto S. Vasorelaxant and antihypertensive effects of methanolic extract from roots of Laelia anceps are mediated by calcium-channel antagonism. Fitoterapia. 2010;81(5):350-357.

Rodrigues AM, Guimarães DO, Konno TU, Tinoco LW, Barth T, Aguiar FA, Lopes NP, Leal IC, Raimundo JM, Muzitano MF. Phytochemical study of Tapirira guianensis leaves guided by vasodilatory and antioxidant activities. Molecules. 2017; 22(2):304-316.

Kim B, Lee K, Chinannai KS, Ham I, Bu Y, Kim H, Choi H-Y. Endothelium-independent vasorelaxant effect of Ligusticum jeholense root and rhizoma on rat thoracic aorta. Molecules. 2015;20(6): 10721-10733.

Johnson O, Adedoyin R, Awotidebe T, Mbada C, Otolorin O, Owoseni I. Cardiovascular risk among undergraduates in a Nigerian University. Int J Public Health Epidemiol. 2013;2(5):085-089.

Konta EM, Almeida MR, Amaral CLD, Darin JDC, Rosso VV, Mercadante AZ, Antunes LMG, Bianchi MLP. Evaluation of the antihypertensive properties of yellow passion fruit pulp (Passiflora edulis Sims f. flavicarpa Deg.) in spontaneously hypertensive rats. Phytother Res. 2014;28(1):28-32.

Mykkänen OT, Huotari A, Herzig K-H, Dunlop TW, Mykkänen H, Kirjavainen PV. Wild blueberries (Vaccinium myrtillus) alleviate inflammation and hypertension associated with developing obesity in mice fed with a high-fat diet. PLoS One. 2014;9(12):e114790.

Mota AH. A review of medicinal plants used in therapy of cardiovascular diseases. Int J Pharmacogn Phytochem Res. 2016;8:572-591.

Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J. Global burden of hypertension: Analysis of worldwide data. The Lancet. 2005; 365(9455):217-223.

Ouédraogo WJC, Koala M, Ouédraogo N, Kini BF, Gerbaux P, Bonzi-Coulibaly YL. Total phenolics and total flavonoid contents, antioxidant activity and flavonoids identification by high-performance liquid chromatography–tandem mass spectrometry of Odontonema strictum (Acanthaceae) Leaves. Asian J Plant Sci Res. 2017;7(5): 54-63.

Handler J. Quality of life and antihypertensive drug therapy. J Clin Hypertens. 2005;7(5):274-285.

Ferreira-Filho ES, Arcanjo DDR, Moura LHP, Silva-Filho JCD, Paulino ET, Ribeiro ÊAN, Chaves MH, Oliveira RDCM, Oliveira APD. Antihypertensive and vasorelaxant effects of ethanol extract of stem barks from Zanthoxylum rhoifolium Lam. in rats. Indian J Exp Biol. 2013;51:661-669.

Kini F, Saba A, Tits M, Angelot L, Guissou PI. Analyse par chromatographie et par spectrometrie electronique des extraits de feuilles de Odontonema strictum (Acanthaceae). Mise en evidence de flavonoïdes du type Flavone. J Soc Ouest-Afr Chim. 2008;25:117-123.

Ouedraogo S, Kini F, Serme L, Nikiema J, Traoré A, Guissou P, Ndiaye M, Bucher B, Andriantsitohaina R. Assessment of the hypotensive and vasodilator effects of extract and fractions from Odontonema strictum (Acanthaceae). Ethnopharmacologia. 2005;36:74-77.

Ouédraogo WJC, Dicko C, Kini FB, Bonzi-Coulibaly YL, Dey ES. Enhanced extraction of flavonoids from Odontonema strictum leaves with antioxidant activity using supercritical carbon dioxide fluid combined with ethanol. J Supercrit Fluids. 2018;131:66-71.

Pierre LL, Moses MN. Isolation and characterisation of stigmasterol and β-sitosterol from Odontonema strictum (Acanthaceae). J Innovations Pharm Biol Sci. 2015;2(1):88-96.

Konczak-Islam I, Okuno S, Yoshimoto M, Yamakawa O. Composition of phenolics and anthocyanins in a sweet potato cell suspension culture. Biochem Eng J. 2003;14(3):155-161.

Choi H, Allahdadi KJ, Tostes RC, Webb RC. Augmented S-nitrosylation contributes to impaired relaxation in angiotensin II hypertensive mouse aorta: Role of thioredoxin reductase. J Hypertens. 2011;29(12):2359-2368.

Lee K, Shin MS, Ham I, Choi H-Y. Investigation of the mechanisms of Angelica dahurica root extract-induced vasorelaxation in isolated rat aortic rings. BMC Complement Altern Med. 2015;15(1): 395-403.

Lee K, Ham I, Yang G, Lee M, Bu Y, Kim H, Choi H-Y. Vasorelaxant effect of Prunus yedoensis bark. BMC Complement Altern Med. 2013;13(1):31-37.

Niazmand S, Fereidouni E, Mahmoudabady M, Mousavi SM. Endothelium-independent vasorelaxant effects of hydroalcoholic extract from Nigella sativa seed in rat aorta: The roles of Ca2+. BioMed Res Int.; 2014.

Belemnaba L, Ouedraogo S, Auger C, Chataigneau T, Traoré A, Guissou IP, Lugnier C, Schini-Kerthand VB, Bucher B. Endothelium-independent and endothelium-dependent vasorelaxation by a dichloromethane fraction from Anogeissus leiocarpus (dc) guill. Etperr. (combretaceae): Possible involvement of cyclic nucleotide phosphodiesterase inhibition. Afr J Tradit Complement Altern Med. 2013;10(2):173-179.

Somé A, Belemnaba L, Belemtougri R, Nikiema M, Ouedraogo S. Endothelium dependent and endothelium independent activity of ethanolic extract of Moringa oleifera Lam. (Moringaceae) on porcine coronary arteries and its underlying mechanisms of vasorelaxation. J Pharmacogn Phytochem. 2016;5(6):259-264.

Belemnaba L, Ouédraogo S, Nitiéma M, Chataigneau T, Guissou IP, Schini-Kerth VB, Bucher B, Auger C. An aqueous extract of the Anogeissus leiocarpus bark (AEAL) induces the endothelium-dependent relaxation of porcine coronary artery rings involving predominantly nitric oxide. J Basic Clin Physiol Pharmacol; 2018.

Belemnaba L, Nitiéma M, Ouédraogo S, Auger C, Schini-Kerth VB, Bernard B. Endothelium-independent vasorelaxation by dichloromethanolic fraction from Anogeissus leiocarpa (DC) Guill. Et Perr. (Combretaceae) bark of trunk on porcine coronary artery rings: Involvement of [Ca2+]i decreased and phosphodiesterases inhibition. Afr J Pharm Pharmacol. 2019;13(4):25-35.

Panthiya L, Pantan R, Tocharus J, Nakaew A, Suksamrarn A, Tocharus C. Endothelium-dependent and endothelium-independent vasorelaxant effects of tiliacorinine 12′-O-acetate and mechanisms on isolated rat aorta. Biomed Pharmacother. 2019;109:2090-2099.

Geleta B, Makonnen E, Debella A, Abebe A, Fekadu N. In vitro vasodilatory activity and possible mechanisms of the crude extracts and fractions of Moringa stenopetala (Baker f.) Cufod. leaves in isolated thoracic aorta of guinea pigs. J Exp Pharmacol. 2016;8:35.

Basri DF, Rahman NSaA, Ali SS, Zainalabidin S. The vasorelaxant effect of Canarium odontophyllum Miq. (Dabai) extract in rat thoracic aorta. Egyptian J Basic Applied Sci. 2018;5(1):75-79.

Kang YH, Shin HM. Cinnamomi ramulus ethanol extract exerts vasorelaxation through inhibition of influx and release in rat aorta. Evidence-Based Complement Altern Med.; 2012.

Getiye Y, Tolessa T, Engidawork E. Antihypertensive activity of 80% methanol seed extract of Calpurnia aurea (Ait.) Benth. subsp. aurea (Fabaceae) is mediated through calcium antagonism induced vasodilation. J Ethnopharmacol. 2016;189:99-106.

Wisutthathum S, Chootip K, Martin H, Ingkaninan K, Temkitthawon P, Totoson P, Demougeot C. Vasorelaxant and hypotensive effects of an ethanolic extract of Eulophia macrobulbon and its main compound (1-(4'-hydroxybenzyl)-4,8-dimethoxyphenanthrene-2,7-diol). Front Pharmacol. 2018;9:484.

Nitiéma M, Soleti R, Koffi C, Belemnaba L, Mallegol P, Ouédraogo N, Kini FB, Ouédraogo S, Guissou IP, Andriantsitohaina R. Ethyl acetate fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) trunk barks corrects angiotensin II-induced hypertension and endothelial dysfunction in mice. Oxid Med Cell Longevity. 2019;13.

Chabert P, Akhtar M, Jabeen Q, Delecolle J, Heintz D, Garo E, Hamburger M, Auger C, Lugnier C, Kim H-J. Endothelium-independent vasorelaxant effect of a Berberis orthobotrys root extract via inhibition of phosphodiesterases in the porcine coronary artery. Phytomedicine. 2016;23(8):793-799.

Sargazi Zadeh G, Panahi N. Endothelium-independent vasorelaxant activity of Trachyspermum ammi essential oil on rat aorta. Clin Exp Hypertens. 2017;39(2):133-138.

Kwan C. The effects of different ginseng extracts on vascular contraction in vitro: Evidence for Yin-Yang principle. Acta Phytother. 1999;2:73-77.

Lih-Geeng C, Yeun-Chin L, Chia-Weu H, Being-Chyuan L, Being-Sun W. Tannin 1-α-O-galloylpunicalagin inducesthe calcium-dependent activation of endothelial nitric-oxide synthase via the phosphatidylinositol 3-kinase/Akt pathway in endothelial cells. Mol Nutr Food Res. 2008;52(10):1162-1171.

Castro-Ruiz JE, Rojas-Molina A, Luna-Vázquez FJ, Rivero-Cruz F, García-Gasca T, Ibarra-Alvarado C. Affinin (Spilanthol), isolated from Heliopsis longipes, Induces vasodilation via activation of gasotransmitters and prostacyclin signaling pathways. Int J Mol Sci. 2017;18(1):218.

Marius L, Rakiatou T, Noufou O, Félix BK, Andre T, Pierre D, Pierre GI. In vitro antioxidant activity and phenolic contents of different fractions of ethanolic extract from Khaya senegalensis A. Juss. (Meliaceae) stem barks. Afr J Pharm Pharmacol. 2016;10(23):501-507.

Costa AGV, Garcia-Diaz DF, Jimenez P, Silva PI. Bioactive compounds and health benefits of exotic tropical red-black berries. J Funct Foods. 2013;5(2):539- 549.

Zhang Y-J, Gan R-Y, Li S, Zhou Y, Li A-N, Xu D-P, Li H-B. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules. 2015;20(12):21138-21156.