Nutraceutical Potential and Antipsychotic Activity of Local Dry Fruit Waste – Juglans regia

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Rachna Shashikant Jadhav
Shanooba Palamthodi
Bhaskar Saha


Aim: To identify the biologically active components in shells of Juglans regia and study its nutraceutical potential and antipsychotic activity for effective waste management.

Study Design: Biochemical and in vivo analyses of plant extract using established protocols. 

Place and Duration of Study: Sample extraction at Department of Food Science and technology, School of Biotechnology and Bioinformatics, DY Patil deemed to be University, Navi Mumbai, India; sample components identification at Sophisticated Analytical Instrument facility (SAIF), Indian Institute of Technology (IIT), Mumbai, India; and in vivo studies for antipsychotic activity using Caenorhabditis elegans at Department of Life Science and Biochemistry, St. Xavier’s College, Mumbai, India between November 2018 and May 2019.

Methodology: The shells of Juglans regia were milled and the extract was prepared using Soxhlet extraction at 60oC using methanol as solvent. The GCMS analysis of the extract was carried out using a GC JEOL – The Accu TOF. Antipsychotic activity was studied using pharyngeal pumping assay in Caenorhabditis elegans.

Results: GC-MS analysis of methanolic extract of shells of Juglans regia revealed the presence of Tridecanoic acid, Acetoxyacetic acid, nonyl ester, 2-hexenal, 2-ethyl, Eicosanoic acid, phenylmethyl ester, Undecane, Benzeneacetic acid decyl ester, (1-pentyl-allyoxymethoxy-methyl)-benzene), 9,12-octadecadienoic acid(Z,Z), phenylmethyl ester, Benzyl oxytridecanoic acid, 6,9,12- octadecatrienoic acid, phenylmethyl ester (Z,Z), 9- octadecanoic acid (Z), phenylmethyl ester, 9,12,15- octadecatrienoic acid, Z [(trimethyl (sil)oxy, 1 – trimethyl (sily)oxy] ethyl ester (Z,Z,Z). Furthermore, behavioural assay done using Caenorhabditis elegans as a model organism showed that the sample exerted antipsychotic activity at lowest concentration.

Conclusion: The shells of Juglans regia being a natural source, can be used as an alternative to the synthetic antipsychotic drugs that have side effects. Our current work suggests that the walnut shells that end up into trash bins are an excellent source of effective natural biologically active compounds.

Caenorhabditis elegans, nutraceutical, antipsychotic, walnut shells, gas chromatography mass spectrometry, pharyngeal pump.

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How to Cite
Jadhav, R., Palamthodi, S., & Saha, B. (2019). Nutraceutical Potential and Antipsychotic Activity of Local Dry Fruit Waste – Juglans regia. European Journal of Medicinal Plants, 29(2), 1-7.
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Cragg GM, Newman DJ. Natural products: A continuing source of novel drug leads. Biochimica et Biophysica Acta (BBA)-General Subjects. 2013;1830(6):3670-3695.

Hernández-Alonso P, Camacho-Barcia L, Bulló M, Salas-Salvadó J. Nuts and dried fruits: An update of their beneficial effects on type 2 diabetes. Nutrients. 2017;9(7): 673.

Pandey MM, Rastogi S, Rawat AKS. Indian traditional ayurvedic system of medicine and nutritional supplementation. Evidence-Based Complementary and Alternative Medicine. 2013;376327.

George S, Muralidhara M. Exploring the role of “Brahmi” (Bocopa monnieri and Centella asiatica) in Brain Function and Therapy. Recent Patents on Endocrine Metabolic & Immune Drug Discovery. 2011;5(1):3349.

Karakaş FP, Coşkun H, Sağlam K, Bozat BG. Lycium barbarum L. (goji berry) fruits improve anxiety, depression-like behaviors, and learning performance: The moderating role of sex. Turkish Journal of Biology. 2016;40(4):762-771.

Ebbo AA, Elsa AT, Etuk EU, Ladan MJ, Saganuwan SA. Antipsychotic effect of aqueous stem bark extract of Amblygonocarpus andongensis in wistar albino rats. Journal of Medicinal Plant Research. 2012;4(11):1033–1038.

Zuardi AW, Crippa JAS, Hallak JEC, Moreira FA, Guimarães FS. Cannabidiol, a Cannabis sativaconstituent, as an antipsychotic drug. Brazilian Journal of Medical and Biological Research. 2006; 39(4):421-429.

Raley-Susman KM, Chou E, Lemoine H. Use of the model organism caenorhabditis elegans to elucidate neurotoxic and behavioral effects of commercial fungicides. Neurotoxins. 2018:37.

Engleman EA, Katner SN, Neal-Beliveau BS. Caenorhabditis elegans as a model to Study the molecular and genetic mechanisms of drug addiction. Progress in Molecular Biology and Translational Science. 2015;137.
[ISSN 1877-1173]

Donohoea DR, Raymond AJ, Kathrine W, Eric JA, Donard SD. Behavioural adaptation in C. Elegans produced by antipsychotic drugs requires serotonin and is associated with calcium signaling and calcineurin inhibition. Journal Neuroscience Research. 2009;64(3):280–289.

Rayomand E. Now, kashmir’s discarded walnut shells can be used to make batteries; 2017. Available:

Chongxi Y. U.S. Patent No. US 20110269689A1, Washington, DC: U.S. Patent and Trademark Office. United States Patent Application Publication; 2011.

Pub. No.: US 2011/0269689

Sommer IE, De Witte L, Begemann M, Kahn RS. Non Steroidal Anti-inflammatory drugs in Schizophrenia: Ready for practice or a good start? A Meta – analysis. Journal of Clinical Psychiatry. 2012;73(4):414– 419.

Peters D, Sandberg CE. U.S. Patent No. WO 03/042162 A1, Washington, DC: U.S. Patent and Trademark Office; 2002.

Sawin ER, Ranganathan R, Horvitz HR. C. elegans locomotory rate is modulated by the environment through a dopaminergic pathway and by experience through a serotonergic pathway. 2000;26(3):619-631.